Recombinant Mouse Thrombopoietin protein (Thpo), partial (Active)

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中文名稱：

小鼠Thpo重組蛋白
品名簡(jiǎn)稱：

Recombinant Mouse Thpo protein, partial (Active)
貨號(hào)：

CSB-AP003451MO
說明書：

MSDS Datasheet
規(guī)格：

￥2124
圖片：
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>95% as determined by SDS-PAGE.
內(nèi)毒素：

Less than 1.0 EU/μg as determined by LAL method.
生物活性：

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human MO7e cells is less than 1.0 ng/ml, corresponding to a specific activity of >1.0x10⁶ IU/mg.
基因名：

Thpo
Uniprot No.：

P40226
別名：

Thpo; Thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Partial
來源：

E.Coli
分子量：

18.7 kDa
表達(dá)區(qū)域：

22-195aa
氨基酸序列

SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF
蛋白標(biāo)簽：

Tag-Free
產(chǎn)品提供形式：

Liquid or Lyophilized powder
緩沖液：

0.2 μm filtered PBS, pH 7.4, with 5% Trehalose ,lyophilized
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

5-10 business days
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
基因功能參考文獻(xiàn)：
1. Increased TPO expression in HSCs following IL-11 exposure could be mimicked or blocked by inhibiting or overexpressing miR-204-5p, respectively... IL-11 promoted Hematopoietic stem cell engraftment in a mouse model of aplastic anemia an effect that was attenuated in cells overexpressing miR-204-5p. PMID: 29217821
2. this study assessed the physiological source of thrombopoietin, a key cytokine required for maintaining hematopoietic stem cell . PMID: 29622652
3. TPO and its receptor Mpl are dispensable for platelet survival in adult mice PMID: 27344013
4. Furthermore, although the contribution of the TPO treated graft to long-term hematological engraftment was reduced, the TPO treated and untreated grafts both contributed significantly to long-term chimerism in vivo. PMID: 25137252
5. TPO treatment also promoted the peripheral induction of Foxp3(+) Tregs in conjunction with elevated circulating TGF-beta levels. PMID: 25212676
6. novel findings about aspects of TPO action on stem cells PMID: 25564715
7. Mpl expression, but not Tpo, is fundamental in the development of JAK2V617F(+) myeloproliferative neoplasms PMID: 25339357
8. Thrombopoietin/MPL signaling confers growth and survival capacity to CD41-positive cells in a mouse model of Evi1 leukemia. PMID: 25298035
9. Megakaryocytes regulate cell cycle quiescence of hematopoietic stem cell through the production of thrombopoietin. PMID: 25451253
10. IEX-1 has a role in activation of Erk and NF-kB pathways, which affects thrombopoietin-promoted NHEJ DNA repair in hematopoietic stem cells PMID: 24184684
11. Findings establish that Clock regulates Thpo and Mpl expression in vivo, and demonstrate an important link between the body's circadian timing mechanisms and megakaryopoiesis. PMID: 22284746
12. Signal transduction pathway of ERK1/2 participates in the activation of thrombopoietin in inflammatory injury of BV2 cells. PMID: 21507308
13. The implication of Tpo as a mediator of neuronal damage in experimental pneumococcal meningitis is reported. PMID: 21149592
14. Administration of recombinant human IL11 after supralethal radiation exposure promotes survival in mice: interactive effect with thrombopoietin PMID: 12005542
15. glycan domain of thrombopoietin acts in trans to enhance secretion of the hormone and other cytokines PMID: 12101178
16. Thrombopoietin expands hematopoietic stem cells after transplantation PMID: 12163458
17. p38 mitogen-activated protein kinase (MAPK) was responsible for the TPO-induced Hoxb4 elevation PMID: 12855555
18. Mpl stimulation by TPO results in the activation of Lyn kinase, which appears to limit the proliferative response through a signaling cascade that regulates MAPK activity PMID: 14726379
19. Thrombopoietin induces HOXA9 nuclear transport in immature hematopoietic cells PMID: 15254242
20. mpl-tpo signaling pathways are negatively modulated by lnk PMID: 15337790
21. is strongly proapoptotic in the brain, causing death of newly generated neurons through the Ras-extracellular signal-regulated kinase 1/2 pathway PMID: 15642952
22. TPO(high) and GATA-1(low) alterations are linked in an upstream-downstream relationship along a pathobiologic pathway leading to development of myelofibrosis PMID: 15665119
23. Development of myelofibrosis and osteosclerosis depends on local TPO levels in Bone marrow cells. PMID: 15927672
24. Thrombopoietin synthesis is induced by IL-6 in the liver during acute inflammation. PMID: 16022585
25. results suggest that a balance in positive and negative signals downstream from the TPO signal plays a role in the regulation of the probability of self-renewal in HSCs PMID: 17284614
26. thrombopoietin has a role in mast cell differentiation [review] PMID: 17468237
27. Chronic TPO overexpression induces mesangioproliferative glomerulopathy. PMID: 17546634
28. roles for FAK in megakaryocyte growth and platelet function, setting the stage for manipulation of this component of the Tpo signaling apparatus for therapeutic benefit. PMID: 17925492
29. Transcriptional regulation of bone marrow thrombopoietin by platelet proteins.( PMID: 18410987
30. TPO induces HIF-1alpha expression in a manner very similar to that of hypoxia. PMID: 18473128
31. thrombopoietin potentiates vasculogenesis by enhancing motility and enlivenment of transplanted endothelial progenitor cells via activation of Akt/mTOR/p70S6kinase signaling pathway PMID: 18773906
32. TPO downmodulates c-Myb expression through induction of miR-150. PMID: 18814950
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亞細(xì)胞定位：

Secreted.
蛋白家族：

EPO/TPO family
組織特異性：

Found mainly in the liver, kidney and skeletal muscle.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:21832

STRING: 10090.ENSMUSP00000075756

UniGene: Mm.3943