Guinea pig monocyte chemotactic protein 1,MCP1 ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豚鼠單核細胞趨化蛋白1(MCP-1/CCL2/MCAF)酶聯免疫試劑盒（CSB-E13559Gp）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CCL2含量。CCL2是一種趨化因子，在免疫調節、炎癥反應等生理病理過程中扮演重要角色。研究發現它能募集單核細胞等免疫細胞到炎癥部位。其異常表達與腫瘤進展、心血管疾病等密切相關，探索CCL2作用機制有助于疾病治療新策略的開發。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，可應用于炎癥機制研究、免疫調節實驗、疾病模型建立等基礎研究領域。例如在豚鼠感染模型或自身免疫性疾病模型中，可通過監測血清或組織樣本中MCP - 1的動態變化，揭示疾病進程中趨化因子的調控規律。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CCL2 ELISA Kit; MCP1 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
縮寫：

CCL2
Uniprot No.：

Q08782
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of guinea pig MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	93	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
亞細胞定位：

Secreted.
蛋白家族：

Intercrine beta (chemokine CC) family
數據庫鏈接：

STRING: 10141.ENSCPOP00000012126