Human Chordin-like protein 1(CHRDL1) ELISA kit

Human Chordin-like protein 1(CHRDL1) ELISA kit

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中文名稱：

人腱蛋白樣蛋白1(CHRDL1)酶聯免疫試劑盒
貨號：

CSB-EL005378HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

人腱蛋白樣蛋白1(CHRDL1)酶聯免疫試劑盒（CSB-EL005378HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CHRDL1含量。人腱蛋白樣蛋白1（CHRDL1）是一種與BMP4結合的拮抗劑，能阻斷其受體相互作用，將神經干細胞從膠質生成重定向到神經發生，支持大腦中的神經元分化。研究顯示，CHRDL1在背腹軸形成、胚胎骨發育和視網膜血管生成中發揮重要作用，并通過調節BMP4的作用來調節視網膜血管生成。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，支持科研人員開展CHRDL1在神經生物學、骨骼發育、腫瘤微環境調控等領域的機制研究，尤其適用于探究CHRDL1在疾病模型中的動態表達及其與BMP通路互作的分子機制。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CHL ELISA Kit; chordin like 1 ELISA Kit; Chordin-like protein 1 ELISA Kit; CHRDL1 ELISA Kit; CRDL1_HUMAN ELISA Kit; dA141H5.1 ELISA Kit; neuralin 1 ELISA Kit; Neuralin-1 ELISA Kit; Neurogenesin-1 ELISA Kit; NRLN1 ELISA Kit; Ventroptin ELISA Kit; VOPT ELISA Kit
縮寫：

CHRDL1
Uniprot No.：

Q9BU40
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-93
1:2	Average %	104
	Range %	98-107
1:4	Average %	96
	Range %	88-99
1:8	Average %	96
	Range %	91-99

回收率：

The recovery of human CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	99	94-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.551	2.487	2.519	2.354
5	2.164	2.112	2.138	1.973
2.5	1.651	1.688	1.670	1.505
1.25	1.043	1.032	1.038	0.873
0.625	0.660	0.645	0.653	0.488
0.312	0.474	0.467	0.471	0.306
0.156	0.268	0.272	0.270	0.105
0	0.163	0.167	0.165

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development.
基因功能參考文獻：
1. Finally, in vivo experiments confirmed that CHRDL1 acted as a tumor suppressor gene in suppressing tumor growth and metastasis PMID: 28423564
2. Results show that Chrdl1 expression is upregulated in numerous breast cancer cells following TGF-beta stimulation and demonstrated that Chrdl1 acts as an inhibitor of BMP4-induced migration and invasion. PMID: 26976638
3. These studies have identified and validated new factors enriched in juvenile chondrocytes as compared to adult chondrocytes including secreted extracellular matrix factors chordin-like 1 (CHRDL1) and microfibrillar-associated protein 4 (MFAP4). PMID: 26955889
4. We provide the initial confirmation that X-linked megalocornea is associated with mutations in the CHRDL1 gene. PMID: 24073597
5. CHRDL1 plays a key role in cornea homeostasis as evidenced by disease causing mutations in X-linked megalocornea. PMID: 25712132
6. The detection of mutations in the CHRDL1 gene is useful for differential diagnosis with different forms of megalocornea. PMID: 26020825
7. Novel CHRDL1 mutations in ten families with X-linked megalocornea, are reported. PMID: 25093588
8. CHRDL1 is expressed in the developing human cornea and anterior segment in addition to the retina. PMID: 22284829
9. Hypoxia-induced upregulation of CHL-1 alters the homeostatic balance between BMP-4 and VEGF to synergize with VEGF in driving retinal angiogenesis. PMID: 18587495
10. reports the cloning of chick ventroptin and its importance in topographic retinotectal projection PMID: 11441185
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相關疾病：

Megalocornea 1, X-linked (MGC1)
亞細胞定位：

Secreted.
組織特異性：

Expressed in the developing cornea and in the eye anterior segment in addition to the retina. Differentially expressed in the fetal brain. There is high expression in cerebellum and neocortex. Expressed in retinal pericytes.
數據庫鏈接：

HGNC: 29861

OMIM: 300350

KEGG: hsa:91851

STRING: 9606.ENSP00000361112

UniGene: Hs.496587

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.