Human Histone H3.1(HIST1H3A) ELISA kit
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中文名稱：人組蛋白H3.1(HIST1H3A)酶聯(lián)免疫試劑盒
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貨號：CSB-EL010418HU
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規(guī)格：96T/48T
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價格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人組蛋白H3.1(HIST1H3A)酶聯(lián)免疫試劑盒（CSB-EL010418HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的HIST1H3A含量。HIST1H3A是一種組蛋白基因。它在染色質(zhì)結(jié)構(gòu)和基因表達(dá)調(diào)控中起關(guān)鍵作用。研究中，其與細(xì)胞增殖、分化等密切相關(guān)。異常表達(dá)可能影響細(xì)胞正常生理功能，引發(fā)疾病，目前對其作用機(jī)制深入研究有助于探索相關(guān)疾病治療靶點(diǎn)。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，支持表觀遺傳學(xué)、疾病分子機(jī)制、藥物篩選等基礎(chǔ)研究，例如探究H3.1在腫瘤模型中的動態(tài)修飾、細(xì)胞周期相關(guān)蛋白互作或特定病理狀態(tài)下組蛋白表達(dá)譜變化。其兼容多種生物樣本的特性，為體外實(shí)驗與體內(nèi)研究的數(shù)據(jù)關(guān)聯(lián)分析提供便利，適用于分子生物學(xué)、細(xì)胞生物學(xué)等領(lǐng)域的定量研究需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：H3 histone family member E pseudogene ELISA Kit; H3 histone family; member A ELISA Kit; H3/A ELISA Kit; H31_HUMAN ELISA Kit; H3F3 ELISA Kit; H3FA ELISA Kit; Hist1h3a ELISA Kit; HIST1H3B ELISA Kit; HIST1H3C ELISA Kit; HIST1H3D ELISA Kit; HIST1H3E ELISA Kit; HIST1H3F ELISA Kit; HIST1H3G ELISA Kit; HIST1H3H ELISA Kit; HIST1H3I ELISA Kit; HIST1H3J ELISA Kit; HIST3H3 ELISA Kit; histone 1; H3a ELISA Kit; Histone cluster 1; H3a ELISA Kit; Histone H3 3 pseudogene ELISA Kit; Histone H3.1 ELISA Kit; Histone H3/a ELISA Kit; Histone H3/b ELISA Kit; Histone H3/c ELISA Kit; Histone H3/d ELISA Kit; Histone H3/f ELISA Kit; Histone H3/h ELISA Kit; Histone H3/i ELISA Kit; Histone H3/j ELISA Kit; Histone H3/k ELISA Kit; Histone H3/l ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates, cell lysates
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檢測范圍：23.5 pg/mL-1500 pg/mL
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靈敏度：5.8 pg/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Epigenetics and Nuclear Signaling
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human HIST1H3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 84 Range % 80-93 1:2 Average % 88 Range % 80-95 1:4 Average % 90 Range % 85-98 1:8 Average % 95 Range % 90-102 -
回收率：
The recovery of human HIST1H3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 90-105 EDTA plasma (n=4) 92 87-97 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1500 2.157 2.134 2.146 2.004 750 1.623 1.562 1.593 1.451 375 1.158 1.082 1.120 0.978 187.5 0.746 0.697 0.722 0.580 94 0.471 0.451 0.461 0.319 47 0.348 0.321 0.335 0.193 23.5 0.253 0.249 0.251 0.109 0 0.148 0.136 0.142 -
數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評價
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靶點(diǎn)詳情
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功能：Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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基因功能參考文獻(xiàn)：
- Data indicate the mechanism for epigenetic regulation in cancer by inducing E3 ubiquitin ligase NEDD4-dependent histone H3 ubiquitination. PMID: 28300060
- the identification of increased expression of H3K27me3 during a patient's clinical course can be helpful for determining whether the tumors are heterochronous PMID: 29482987
- Here, we report that JMJD5, a Jumonji C (JmjC) domain-containing protein, is a Cathepsin L-type protease that mediates histone H3 N-tail proteolytic cleavage under stress conditions that cause a DNA damage response. PMID: 28982940
- Data suggest that Ki-67 antigen proliferative index has important limitations and hhosphohistone H3 (PHH3) is an alternative proliferative marker. PMID: 29040195
- These results identify cytokine-induced histone 3 lysine 27 trimethylation as a mechanism that stabilizes gene silencing in macrophages PMID: 27653678
- This data indicates that, in the early developing human brain, HIST1H3B constitutes the largest proportion of H3.1 transcripts among H3.1 isoforms. PMID: 27251074
- This series of 47 diffuse midline gliomas, histone H3-K27M mutation was mutually exclusive with IDH1-R132H mutation and EGFR amplification, rarely co-occurred with BRAF-V600E mutation, and was commonly associated with p53 overexpression, ATRX loss, and monosomy 10. Among these K27M+ diffuse midline gliomas. PMID: 26517431
- Data show that histone chaperone HIRA co-localizes with viral genomes, binds to incoming viral and deposits histone H3.3 onto these. PMID: 28981850
- These experiments showed that PHF13 binds specifically to DNA and to two types of histone H3 methyl tags (lysine 4-tri-methyl or lysine 4-di-methyl) where it functions as a transcriptional co-regulator. PMID: 27223324
- Hemi-methylated CpGs DNA recognition activates UHRF1 ubiquitylation towards multiple lysines on the H3 tail adjacent to the UHRF1 histone-binding site. PMID: 27595565
- We describe, for the first time, the MR imaging features of pediatric diffuse midline gliomas with histone H3 K27M mutation PMID: 28183840
- Approximately 30% of pediatric high grade gliomas (pedHGG) including GBM and DIPG harbor a lysine 27 mutation (K27M) in histone 3.3 (H3.3) which is correlated with poor outcome and was shown to influence EZH2 function. PMID: 27135271
- H3F3A K27M mutation in adult cerebellar HGG is not rare. PMID: 28547652
- Data show that lysyl oxidase-like 2 (LOXL2) is a histone modifier enzyme that removes trimethylated lysine 4 (K4) in histone H3 (H3K4me3) through an amino-oxidase reaction. PMID: 27735137
- Histone H3 lysine 9 (H3K9) acetylation was most prevalent when the Dbf4 transcription level was highest whereas the H3K9me3 level was greatest during and just after replication. PMID: 27341472
- SPOP-containing complex regulates SETD2 stability and H3K36me3-coupled alternative splicing. PMID: 27614073
- Data suggest that binding of helical tail of histone 3 (H3) with PHD ('plant homeodomain') fingers of BAZ2A or BAZ2B (bromodomain adjacent to zinc finger domain 2A or 2B) requires molecular recognition of secondary structure motifs within H3 tail and could represent an additional layer of regulation in epigenetic processes. PMID: 28341809
- The results demonstrate a novel mechanism by which Kdm4d regulates DNA replication by reducing the H3K9me3 level to facilitate formation of preinitiation complex. PMID: 27679476
- Histone H3 modifications caused by traffic-derived airborne particulate matter exposures in leukocytes PMID: 27918982
- A key role of persistent histone H3 serine 10 or serine 28 phosphorylation in chemical carcinogenesis through regulating gene transcription of DNA damage response genes PMID: 27996159
- hTERT promoter mutations are frequent in medulloblastoma and are associated with older patients, prone to recurrence and located in the right cerebellar hemisphere. On the other hand, histone 3 mutations do not seem to be present in medulloblastoma. PMID: 27694758
- AS1eRNA-driven DNA looping and activating histone modifications promote the expression of DHRS4-AS1 to economically control the DHRS4 gene cluster. PMID: 26864944
- Data suggest that nuclear antigen Sp100C is a multifaceted histone H3 methylation and phosphorylation sensor. PMID: 27129259
- The authors propose that histone H3 threonine 118 phosphorylation via Aurora-A alters the chromatin structure during specific phases of mitosis to promote timely condensin I and cohesin disassociation, which is essential for effective chromosome segregation. PMID: 26878753
- Hemi-methylated DNA opens a closed conformation of UHRF1 to facilitate its H3 histone recognition. PMID: 27045799
- Functional importance of H3K9me3 in hypoxia, apoptosis and repression of APAK. PMID: 25961932
- Taken together, the authors verified that histone H3 is a real substrate for GzmA in vivo in the Raji cells treated by staurosporin. PMID: 26032366
- We conclude that circulating H3 levels correlate with mortality in sepsis patients and inversely correlate with antithrombin levels and platelet counts. PMID: 26232351
- Data show that double mutations on the residues in the interface (L325A/D328A) decreases the histone H3 H3K4me2/3 demethylation activity of lysine (K)-specific demethylase 5B (KDM5B). PMID: 24952722
- Data indicate that minichromosome maintenance protein 2 (MCM2) binding is not required for incorporation of histone H3.1-H4 into chromatin but is important for stability of H3.1-H4. PMID: 26167883
- Data suggest that histone H3 lysine methylation (H3K4me3) serves a crucial mechanistic role in leukemia stem cell (LSC) maintenance PMID: 26190263
- PIP5K1A modulates ribosomal RNA gene silencing through its interaction with histone H3 lysine 9 trimethylation and heterochromatin protein HP1-alpha. PMID: 26157143
- Data indicate that the lower-resolution mass spectrometry instruments can be utilized for histone post-translational modifications (PTMs) analysis. PMID: 25325711
- Data indicate that inhibition of lysine-specific demethylase 1 activity prevented IL-1beta-induced histone H3 lysine 9 (H3K9) demethylation at microsomal prostaglandin E synthase 1 (mPGES-1) promoter. PMID: 24886859
- The authors report that de novo CENP-A assembly and kinetochore formation on human centromeric alphoid DNA arrays is regulated by a histone H3K9 acetyl/methyl balance. PMID: 22473132
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相關(guān)疾病：Glioma (GLM)
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亞細(xì)胞定位：Nucleus. Chromosome.
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蛋白家族：Histone H3 family
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