Human endothelial cell-specific molecule 1(ESM-1) ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述：
人內(nèi)皮細(xì)胞特異分子-1(ESM-1)酶聯(lián)免疫試劑盒（CSB-E16530h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿s.樣本中的ESM1含量。試劑盒檢測(cè)范圍為3.12 ng/ml-200 ng/ml，靈敏度為0.78 ng/ml。可廣泛應(yīng)用于心血管疾病機(jī)制、腫瘤微環(huán)境研究、炎癥相關(guān)疾病模型等科研領(lǐng)域，為探索ESM - 1在血管內(nèi)皮功能調(diào)控、腫瘤血管新生等過(guò)程中的作用提供可靠工具，助力生物標(biāo)志物篩選及分子機(jī)制研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
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別名：
Endocan ELISA Kit; Endothelial cell specific molecule 1 ELISA Kit; Endothelial cell-specific molecule 1 ELISA Kit; ESM 1 ELISA Kit; ESM 1 secretory protein ELISA Kit; ESM-1 ELISA Kit; Esm1 ELISA Kit; ESM1 secretory protein ELISA Kit; ESM1_HUMAN ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, tissue homogenatess.
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檢測(cè)范圍：
3.12 ng/ml-200 ng/ml.
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靈敏度：
0.78 ng/ml
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反應(yīng)時(shí)間：
1-5h
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樣本體積：
50-100ul
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檢測(cè)波長(zhǎng)：
450 nm
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研究領(lǐng)域：
Tags & Cell Markers
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測(cè)定原理：
quantitative
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測(cè)定方法：
Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human ESM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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| Sample | Serum(n=4) | |
1:1 | Average % | 100 | |
Range % | 96-104 | |
1:2 | Average % | 106 | |
Range % | 102-112 | |
1:4 | Average % | 97 | |
Range % | 90-104 | |
1:8 | Average % | 101 | |
Range % | 97-105 | |
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回收率：
The recovery of human ESM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type | Average % Recovery | Range | |
Serum (n=5) | 102 | 99-105 | |
EDTA plasma (n=4) | 98 | 91-105 | |
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標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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ng/ml | OD1 | OD2 | Average | Corrected | |
200 | 1.674 | 1.634 | 1.654 | 1.464 | |
100 | 1.275 | 1.247 | 1.261 | 1.070 | |
50 | 0.983 | 1.028 | 1.005 | 0.815 | |
25 | 0.727 | 0.739 | 0.733 | 0.543 | |
12.5 | 0.595 | 0.621 | 0.608 | 0.417 | |
6.25 | 0.440 | 0.453 | 0.447 | 0.256 | |
3.12 | 0.316 | 0.304 | 0.310 | 0.120 | |
0 | 0.186 | 0.195 | 0.191 | | |
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human ESM1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled ESM1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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數(shù)據(jù)處理：
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貨期：
3-5 working days
引用文獻(xiàn)
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Melatonin partly reverses differentiation and apoptosis resistance caused by chemical hypoxia
X LIU, HUI LIU, Y QI, S ZHANG, Y MA,Nusantara Bioscience,2025
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Olink Profiling of Intestinal Tissue Identifies Novel Biomarkers For Colorectal Cancer
C Xiao, H Wu, J Long, F You, X Li,Journal of Proteome Research,2025
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Diagnostic Utility of Endocan and Interleukins for Late-Onset Neonatal Sepsis
PR Gatseva,/,2023
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Blood Endocan as a Biomarker for Breast Cancer Recurrence
J Lv,/,2024
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Early Diagnostic Markers of Late-Onset Sepsis of the Newborn
PR Gatseva,/,2023
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Is Endocan a Novel Diagnostic Marker for the Severity of Bronchopulmonary Dysplasia in Very Low Birth Weight Infants?
CuneytTayman, et al,Archivos de bronconeumolog鉚a,2019
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Is Serum Endocan Levels Important in Branch Retinal Vein Occlusion?
KURT, Ali, et al,Retina-vitreus/journal of retina-vitreous,2018
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The Relationship Between Serum Endocan Level and Aortic Elastic Properties in Patients With Newly Diagnosed Essential Hypertension
Celik M, et al,Angiology,2019
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Endocan is a predictor of increased cardiovascular risk in women with polycystic ovary syndrome
M Bicer.et al,Endocrine Research ,2016
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Elevated endocan concentration is associated with coronary slow flow
Ye MF.et al,Scand J Clin Lab Invest.,2016
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Elevated Human Endothelial Cell-Specific Molecule-1 Level and Its Association With Coronary Artery Disease in Patients With Hypertension.
Xiong C. et al,J Investig Med.,2015
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Tumor necrosis factor superfamily member 15 (Tnfsf15) facilitates lymphangiogenesis via upregulation of Vegfr3 gene expression in lymphatic endothelial cells
Qin TT. et al,J Pathol,2015
靶點(diǎn)詳情
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功能：
Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions.
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基因功能參考文獻(xiàn)：
- Results show that aerum ESM-1 levels were significantly higher in the idiopathic sudden sensorineural hearing loss group than the control group which may confirm endothelial dysfunction involvement. PMID: 30370871
- suggest that measurement of endocan level may help clinicians to predict the development of coronary collateral circulation in patients with stable coronary artery disease PMID: 28393589
- suggest that serum ESM-1 level may be a novel endothelial dysfunction biomarker PMID: 26927690
- Plasma ESM-1 levels are decreased during pregnancy and increased in early- and late-onset preeclampsia. The source of ESM-1 is probably not the placenta, but most likely maternal endothelial cells. PMID: 29674200
- Serial endocan concentration meets the major requirements for predicting outcome in patients with severe sepsis. An assay of endocan concentration may be a good prognostic biomarker in the clinic for severe sepsis. PMID: 29750963
- The cutoff serum level of endocan predicting AD was >288.94 ng/mL in receiver operating characteristic curve analysis PMID: 29393258
- A strong negative correlation was found between serum endocan levels and peak systolic velocity in men with severe erectile dysfunction. PMID: 29057489
- Results show the endocan level in peripheral blood significantly elevated in patients newly diagnosed with multiple myeloma (MM), and associated with myeloma-specific clinical parameters. PMID: 30194153
- The correlation between the secretion of endocan and that of IL-6 by endothelial cells suggests that endocan can be used as a predictive marker of inflammation for the quality assessment of transfusion grade PLTs PMID: 29238987
- Serum ESM-1 levels correlate with stress hyperglycemia in patients with STEMI and are associated with short-term prognosis. PMID: 26685180
- Endocan could be a common predictor of the endothelium-dependent inflammatory processes, rather than related with specific risk factors in coronary artery disease and microvascular angina. PMID: 26744512
- Endocan is an inflammatory marker released by the endothelium and is associated with endothelial dysfunction and atherosclerosis in patients with systemic lupus erythematosus. PMID: 26614790
- Endocan levels are higher in patients with large-artery atherosclerotic stroke and can help in predicting the short-term unfavourable outcome. PMID: 29287901
- elevated expression on epidermal keratinocytes and dermal endothelial cells in lesional skin of atopic dermatitis; decreased expression in atopic dermatitis sera PMID: 28714079
- Plasma ESM-1 is a novel biomarker for predicting the T status in OSCC patients. PMID: 29104463
- ESM1 expression correlates with Gleason grade/score and with androgen receptor expression in prostate carcinoma tissue, so ESM1 should also be considered as a biomarker for diagnosis of prostate cancer. PMID: 29104483
- Demonstrate an increased Endocan serum level in NAFLD patients, higher in those with type 2 diabetes mellitus and/or NASH because of an endothelial dysfunction in these pathologies. PMID: 28922438
- Studied serum endocan levels in endometrial and ovarian cancer. Serum endocan levels were significantly higher in the entire patient group than in the controls. PMID: 27734523
- This study showed that ESM-1 level in pleural effusion could be a potential diagnostic and prognostic marker in NSCLC patients with MPE. PMID: 28514746
- Severe erectile dysfunction was associated with higher serum endocan levels than less severe erectile dysfunction or healthy controls. PMID: 28424502
- Serum endocan levels >/= 2.05 ng/ml had a sensitivity of 76.1% and specificity of 85% for the diagnosis of bacterial infection in decompensated cirrhotic patients. The endocan measured at admission is a good clinical parameter predicting the occurrence of infection in these patients. Elevated endocan may reflect the degree of endothelial cell injury by a systemic inflammatory response. PMID: 28614777
- study showed that higher endocan levels were significantly and independently related to the presence of slow coronary flow. PMID: 26607436
- The results suggest that endocan may have a role in the pathogenesis of pterygium. PMID: 28410547
- Endocan expression is increased in the human placenta from obese women with gestational diabetes, and in response to pro-inflammatory stimuli. Loss-of-function studies in villous trophoblast cells and HUVECs revealed that endocan is not directly involved in the genesis or in the expression of pro-inflammatory cytokines induced by LPS or IL-1beta. PMID: 27871471
- Systemic inflammation may be responsible for the rise in endocan levels following cardiopulmonary bypass. PMID: 26999704
- Endocan expression was correlated with poor clinical outcomes in patients with Pancreatic Neuroendocrine Tumors . The results indicated that endocan expression may be a reliable marker for predicting tumor recurrence in patients with PNETs. PMID: 29019897
- glomerular-derived Esm-1 as a potential non-invasive biomarker of diabetic nephropathy PMID: 28934365
- Physical activity increases circulating endocan level. PMID: 28056805
- These findings provide the first evidence that the imbalance of MMP-9/TIMP-1, is one of the regulation mechanisms by which ESM1 promotes tumorigenicity and metastasis of prostate cancer cells. PMID: 28108731
- These results are consistent with the view that endocan measured in ICU patients is intact, stable, and accurate. Then, the low endocan level observed in ICU patients who developed acute respiratory distress syndrome is likely to be reliable. PMID: 28427918
- Dopamine agonist resistance-related ESM1 promotes the angiogenesis and tumor cells growth of prolactinomas, suggesting that ESM1 may be a novel therapeutic target for prolactinomas. PMID: 26662185
- These results suggest human cytomegalovirus infection leads to glioma progression through an upregulation of endocan and the secretion of inflammatory cytokines. PMID: 27474433
- Endocan-expressing microvessel density significantly correlates with the expression of VEGF and VEGFR2 and is a valuable prognostic factor for survival in human gastric cancer. PMID: 27340359
- Circulating P-selectin levels were closely associated with endocan levels in obese children and adolescents with non-alcoholic fatty liver disease. PMID: 28318371
- this study shows that endocan may play an important role in the development of connective tissue disease related pulmonary arterial hypertension PMID: 27912147
- The distribution of Gleason score and biochemical failure rate were significantly higher in patients with serum endocan >/=1.8 ng/mL (p = 0.031 and p = 0.047). PMID: 27470607
- endocan plays an anti-carcinogenic role in gastric cancer development and progression and might serve as a prognostic biomarker as well as a potential therapeutic target for gastric cancer. PMID: 27644250
- High ESM1 expression is associated with Meningiomas and Gliomas. PMID: 27528791
- Serum and urinary endocan concentrations are significantly elevated in patients with bladder cancer. PMID: 26748103
- Subclinical hypothyroidism is associated with increased levels of serum endocan, dimethylarginine, and TGF-beta, which are new markers for endothelial dysfunction. PMID: 27993872
- The levels of ESM1, CLDN5, IL-1beta, IL-6, and TNF-alpha were significantly higher in the migraine attack group than in the control group PMID: 27010153
- Results found that serum endocan levels were significantly lower in Alzheimer's disease (AD) patients with depression than in AD patients without depression or healthy controls suggesting that serum endocan levels might be associated with depression in AD. PMID: 26924874
- serum levels of endocan, a new biomarker of endothelial dysfunction, increase in obstructive sleep apnea subjects. PMID: 26808694
- Endocan may play a role in the progression of pregnancy and has a potential to be a new marker for the detection of pre-eclampsia PMID: 26823798
- Serum endocan concentrations were significantly elevated in women with pre-eclampsia versus normotensive controls, and concentrations seem to be associated with the severity of the disease. PMID: 26418319
- Suggest that serum endocan concentrations are elevated in patients with type 2 diabetes and decrease following anti-hyperglycemic treatment. PMID: 25969567
- Median maternal plasma endocan concentrations were higher in preeclampsia and lower in acute pyelonephritis with bacteremia than in uncomplicated pregnancy. PMID: 25211383
- Elevated endocan levels were more strongly associated with disease severity among Johnson Syndrome, Toxic Epidermal Necrolysis subjects than among less severe allergic reactions with skin involvement. PMID: 25394955
- Detection of ESM-1 levels in the serum may serve as a tumor biomarker for assessing prognosis in colorectal cancer. PMID: 26125749
- Serum endocan level is independently correlated with the presence and severity of coronary artery disease in hypertension patients. PMID: 26200037
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亞細(xì)胞定位：
Secreted.
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組織特異性：
Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.
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數(shù)據(jù)庫(kù)鏈接：
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