• A 96-well Assay plate --The 96-well plate has been pre-coated with HS.
• Standard (2 x 250 μl)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
• Anti-human HS Antibody (100 x concentrate) (1 x 60 μl)
• Antibody Diluent (1 x 10 ml) --Dilute the anti-human LRG1
• HRP-conjugated goat-anti-mouse HS antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
• HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated HS antibody.
• Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
• Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
• TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
• Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
• Four Adhesive Strips (For 96 wells)
• An Instruction manual

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• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
• An incubator that can provide stable incubation conditions up to 37°C±5°C.
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate
• 50-300ul multi-channel micropipette
• Pipette tips
• Single-channel micropipette with different ranges
• 100ml and 500ml graduated cylinders
• Deionized or distilled water
• Timer
• Test tubes for dilution

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