Monkey Apolipoprotein E(APOE) ELISA kit

Monkey Apolipoprotein E(APOE) ELISA kit

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中文名稱：

猴載脂蛋白E(APOE)酶聯免疫試劑盒
貨號：

CSB-EL001936RH
規格：

96T/48T
價格：

￥4200/￥3000
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產品描述：

猴載脂蛋白E(APOE)酶聯免疫試劑盒（CSB-EL001936RH）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、唾液、組織勻漿樣本中的APOE含量。APOE 基因編碼載脂蛋白 E，在脂質代謝、膽固醇運輸等過程中發揮重要作用。其與心血管疾病、阿爾茨海默病等密切相關。研究機制主要圍繞其對脂蛋白代謝調控、介導脂質轉運、影響神經細胞功能及炎癥反應等方面展開。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于科研場景中探索APOE在細胞模型、動物實驗或生物樣本中的表達變化，如研究脂質代謝調控機制、神經退行性疾病相關蛋白互作，或通過唾液等非侵入性樣本開展初步篩查；可滿足體外實驗的高通量檢測需求，為生命科學基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

APOEApolipoprotein E ELISA kit; Apo-E ELISA kit
縮寫：

APOE
Uniprot No.：

Q28502
種屬：

Monkey
樣本類型：

serum, plasma, cell culture supernates, saliva, tissue homogenates
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
400	0.078	0.082	0.080
200	0.138	0.143	0.141
100	0.212	0.219	0.216
50	0.375	0.381	0.378
25	0.599	0.634	0.617
12.5	0.816	0.833	0.825
6.25	1.124	1.111	1.118
0	1.956	1.894	1.925

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells. A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes. APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues. By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis. APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis. First, it is functionally associated with ABCA1 in the biogenesis of HDLs in tissues. Second, it is enriched in circulating HDLs and mediates their uptake by hepatocytes. APOE also plays an important role in lipid transport in the central nervous system, regulating neuron survival and sprouting.
亞細胞定位：

Secreted. Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
蛋白家族：

Apolipoprotein A1/A4/E family
數據庫鏈接：

STRING: 9544.ENSMMUP00000018786

UniGene: Mmu.3035

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.