Mouse Cystatin-F(CST7) ELISA kit

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中文名稱：

小鼠胱抑素F(CST7)酶聯(lián)免疫試劑盒
貨號：

CSB-EL006095MO
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

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小鼠胱抑素F(CST7)酶聯(lián)免疫試劑盒（CSB-EL006095MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CST7含量。小鼠胱抑素F（CST7）是一種具有半胱氨酸型內(nèi)肽酶抑制活性的蛋白質(zhì)，參與肽酶活性的負(fù)調(diào)控，在抑制半胱氨酸型內(nèi)肽酶活性、負(fù)調(diào)控小膠質(zhì)細(xì)胞激活和正調(diào)控髓鞘化等過程中發(fā)揮作用，其表達(dá)與神經(jīng)炎癥和神經(jīng)病理性疼痛有關(guān)。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，該產(chǎn)品適用于神經(jīng)科學(xué)、免疫學(xué)等領(lǐng)域的基礎(chǔ)研究，可應(yīng)用于實驗動物模型（如神經(jīng)損傷、炎癥性疾病模型）中胱抑素F的動態(tài)監(jiān)測，為探究其與疾病發(fā)生發(fā)展的關(guān)聯(lián)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Cst7Cystatin-F ELISA Kit; Cystatin-7 ELISA Kit; Cystatin-like metastasis-associated protein ELISA Kit; CMAP ELISA Kit; Leukocystatin ELISA Kit
縮寫：

CST7
Uniprot No.：

O89098
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CST7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-93
1:2	Average %	100
	Range %	95-105
1:4	Average %	87
	Range %	83-90
1:8	Average %	104
	Range %	100-107

回收率：

The recovery of mouse CST7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	95	91-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.547	2.601	2.574	2.452
5	2.120	2.032	2.076	1.954
2.5	1.394	1.402	1.398	1.276
1.25	0.820	0.805	0.813	0.691
0.625	0.517	0.531	0.524	0.402
0.312	0.325	0.326	0.326	0.204
0.156	0.239	0.242	0.241	0.119
0	0.121	0.123	0.122

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Monocytes release cystatin F dimer to associate with Aβ and aggravate amyloid pathology and cognitive deficits in Alzheimer's disease Q Li,Journal of neuroinflammation,2024

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功能：

Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
基因功能參考文獻(xiàn)：
1. High CST7 expression is associated with Alzheimer's disease. PMID: 28904096
2. Our results validate cystatin F as a useful biomarker of early pathogenesis in experimental models of prion disease, and point to unexpected species-specific differences in the transcriptional responses to prion infections. PMID: 28178353
3. During the active remyelinating phase, both CysF knockdown (CysFKD) and microglial-selective CatC overexpression (CatCOE) showed a worsening of the demyelination in Plp(4e/-) transgenic mice. Conversely, during the chronic demyelinating phase, CatC knockdown (CatCKD) ameliorated the demyelination. Our results suggest that the balance between CatC and CysF expression controls the demyelination and remyelination process. PMID: 28251676
4. The cystatin F expression in the activated microglia is closely associated with the effect of the A2A receptors, which may be related to the neuroinflammatory responses occurring during the pathological process. PMID: 23285090
5. an inhibitory activity other than cystatin F quenches CatL activity in activated macrophages PMID: 21956111
6. Expression of cystatin F indicates ongoing demyelination and remyelination; absence of cystatin F expression indicates cessation of remyelination in the demyelinating area. PMID: 21344476
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Cystatin family
數(shù)據(jù)庫鏈接：

KEGG: mmu:13011

STRING: 10090.ENSMUSP00000086606

UniGene: Mm.12965

Mouse Cystatin-F(CST7) ELISA kit

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