Mouse Histone deacetylase complex subunit SAP130(SAP130) ELISA kit

產(chǎn)品詳情

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文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠組蛋白去乙酰化酶復(fù)合物亞基SAP130(SAP130)酶聯(lián)免疫試劑盒（CSB-EL020694MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SAP130含量。試劑盒檢測(cè)范圍為46.88 pg/mL-3000 pg/mL，本產(chǎn)品適用于基礎(chǔ)科研領(lǐng)域，如探究表觀遺傳調(diào)控機(jī)制、建立炎癥相關(guān)疾病動(dòng)物模型、評(píng)估藥物干預(yù)對(duì)SAP130表達(dá)的影響等研究場(chǎng)景，為探索SAP130在免疫調(diào)控和疾病發(fā)生中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Sap130 ELISA Kit; Histone deacetylase complex subunit SAP130 ELISA Kit; 130 kDa Sin3-associated polypeptide ELISA Kit; Sin3-associated polypeptide p130 ELISA Kit
縮寫：

SAP130
Uniprot No.：

Q8BIH0
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

46.88 pg/mL-3000 pg/mL
靈敏度：

11.72 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Epigenetics and Nuclear Signaling
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAP130 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	98
	Range %	93-102
1:4	Average %	93
	Range %	89-98
1:8	Average %	91
	Range %	87-97

回收率：

The recovery of mouse SAP130 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	94	93-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
亞細(xì)胞定位：

Nucleus.
蛋白家族：

SAP130 family
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:269003

STRING: 10090.ENSMUSP00000025109

UniGene: Mm.194538