Pig Angiopoietin 2,ANG-2 ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬血管生成素2(ANG-2)酶聯(lián)免疫試劑盒（CSB-E08460p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ANGPT2含量。ANGPT2是一個備受關注的靶點。它在血管生成、血管穩(wěn)態(tài)維持等方面發(fā)揮重要作用。其研究機制主要圍繞調(diào)控內(nèi)皮細胞功能、影響血管通透性等。針對它的研究有望為腫瘤、心血管疾病等提供新的治療策略和藥物研發(fā)方向。試劑盒檢測范圍為0.94 ng/mL-60 ng/mL，適用于科研場景下豬模型的組織病理分析、血清/血漿循環(huán)標志物動態(tài)監(jiān)測，或藥物干預后血管生成相關通路的機制研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

ANGPT2Angiopoietin-2 ELISA kit; ANG-2 ELISA kit
縮寫：

ANGPT2
Uniprot No.：

Q9BDY7
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.94 ng/mL-60 ng/mL
靈敏度：

0.235 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-89
1:2	Average %	99
	Range %	96-102
1:4	Average %	87
	Range %	84-90
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of pig ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-92
EDTA plasma (n=4)	93	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating angiogenic signals mediated by ANGPT1. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
基因功能參考文獻：
1. expression of angiopoietin 2 in the porcine metanephric kidney was observed in the podocytes of early developing glomeruli, but not in the cells near the glomerular hilus PMID: 20598355
2. ANGPT2 remained upregulated during maturation of glomeruli, which may be explained by the continuous growth of the glomeruli, as observed by stereological examination. PMID: 20713984
亞細胞定位：

Secreted.
數(shù)據(jù)庫鏈接：

KEGG: ssc:396730

STRING: 9823.ENSSSCP00000016694

UniGene: Ssc.240