Pig glial fibrillary acidic protein,GFAP ELISA Kit

Pig glial fibrillary acidic protein,GFAP ELISA Kit

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中文名稱：

豬神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)酶聯(lián)免疫試劑盒
貨號：

CSB-E14950p
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

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產(chǎn)品描述：

豬神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)酶聯(lián)免疫試劑盒（CSB-E14950p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GFAP含量。GFAP（膠質(zhì)纖維酸性蛋白）是一種特定于中樞神經(jīng)系統(tǒng)的III類中間絲蛋白，主要在星形膠質(zhì)細(xì)胞中表達(dá)。其研究機(jī)制涉及基因表達(dá)調(diào)控、蛋白結(jié)構(gòu)、功能以及與多種神經(jīng)系統(tǒng)疾病的關(guān)系，如阿爾茨海默病和亞歷山大病。GFAP在維持星形膠質(zhì)細(xì)胞形態(tài)、血腦屏障形成和突觸功能中發(fā)揮重要作用。試劑盒檢測范圍為0.1 ng/mL-20 ng/mL，本試劑盒適用于豬模型在神經(jīng)退行性疾病研究（如阿爾茨海默病、帕金森?。?、腦創(chuàng)傷模型評估、神經(jīng)毒性實驗或神經(jīng)炎癥機(jī)制探索等科研場景中，定量分析血液或組織樣本的GFAP動態(tài)變化，為神經(jīng)病理學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

GFAP
Uniprot No.：

F1RR02
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.1 ng/mL-20 ng/mL
靈敏度：

0.05 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig GFAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-97
1:2	Average %	96
	Range %	92-99
1:4	Average %	92
	Range %	89-96
1:8	Average %	96
	Range %	90-102

回收率：

The recovery of pig GFAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-105
EDTA plasma (n=4)	96	92-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
20	0.187	0.195	0.191
5	0.338	0.346	0.342
1.6	0.559	0.551	0.555
0.4	0.842	0.868	0.855
0.1	1.334	1.337	1.336
0	1.989	2.005	1.997

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Can Recognition of Spinal Ischemia Be Improved? Application of Motor-Evoked Potentials, Serum Markers, and Breath Gas Analysis in an Acutely Instrumented Pig Model Anja Puschel.et al,ANN VASC SURG,2018
Can recognition of spinal ischemia be improved? Application of motor-evoked potentials, serum markers and breath gas analysis in an acutely instrumented pig model Pschel A.et al,Ann Vasc Surg,2018

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.