Rat Bone morphogenetic protein 4,BMP-4 ELISA Kit

Rat Bone morphogenetic protein 4,BMP-4 ELISA Kit

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中文名稱：

大鼠骨成型蛋白4(BMP-4)酶聯(lián)免疫試劑盒
貨號：

CSB-E04511r
規(guī)格：

96T/48T
價格：

￥3900/￥2500
其他：

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產(chǎn)品描述：

大鼠骨成型蛋白4(BMP-4)酶聯(lián)免疫試劑盒（CSB-E04511r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BMP4含量。BMP4，即骨形態(tài)發(fā)生蛋白4，是TGF-β超家族成員，具有促進成骨、血管生成、胚胎分化和神經(jīng)干細胞分化等功能。其研究背景涉及心血管疾病、骨再生和腫瘤生長等，機制上通過調(diào)節(jié)細胞增殖、分化和遷移等過程發(fā)揮作用。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，本試劑盒適用于科研領(lǐng)域中對BMP - 4功能機制的探索，例如骨骼發(fā)育動態(tài)研究、再生醫(yī)學(xué)中組織修復(fù)模型的分子機制解析、胚胎發(fā)育過程中形態(tài)發(fā)生調(diào)控的定量分析，以及相關(guān)疾病模型的分子標志物檢測。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Bmp4 ELISA Kit; Bmp-4 ELISA Kit; Dvr-4Bone morphogenetic protein 4 ELISA Kit; BMP-4 ELISA Kit; Bone morphogenetic protein 2B ELISA Kit; BMP-2B ELISA Kit
縮寫：

BMP4
Uniprot No.：

Q06826
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-105
1:2	Average %	91
	Range %	85-97
1:4	Average %	86
	Range %	82-90
1:8	Average %	94
	Range %	90-100

回收率：

The recovery of rat BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	92	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.514	2.651	2.583	2.468
250	1.867	1.797	1.832	1.717
125	1.279	1.315	1.297	1.182
62.5	0.885	0.842	0.864	0.749
31.2	0.513	0.538	0.526	0.411
15.6	0.294	0.289	0.292	0.177
7.8	0.201	0.213	0.207	0.092
0	0.112	0.118	0.115

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical BMP pathways such as ERK/MAP kinase, PI3K/Akt, or SRC cascades. For example, induces SRC phosphorylation which, in turn, activates VEGFR2, leading to an angiogenic response.
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

TGF-beta family
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000012957

UniGene: Rn.10318

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.