Rat matrix metalloproteinase 13,MMP-13 ELISA kit

Rat matrix metalloproteinase 13,MMP-13 ELISA kit

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中文名稱：

大鼠基質(zhì)金屬蛋白酶13(MMP-13)酶聯(lián)免疫試劑盒
貨號：

CSB-E07412r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

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產(chǎn)品描述：

大鼠基質(zhì)金屬蛋白酶13(MMP-13)酶聯(lián)免疫試劑盒（CSB-E07412r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP13含量。MMP13即基質(zhì)金屬蛋白酶 13，在人體組織重塑、胚胎發(fā)育等生理過程中發(fā)揮作用，異常表達與關(guān)節(jié)炎、癌癥等疾病相關(guān)。研究聚焦其在疾病發(fā)生發(fā)展中的機制，如促進腫瘤侵襲轉(zhuǎn)移等，旨在開發(fā)針對性治療藥物。試劑盒檢測范圍為93.75 pg/mL-6000 pg/mL，適用于探索骨關(guān)節(jié)炎、腫瘤侵襲轉(zhuǎn)移等疾病模型中基質(zhì)代謝調(diào)控機制，或評估創(chuàng)傷修復(fù)、炎癥微環(huán)境等基礎(chǔ)研究中的蛋白酶活性動態(tài)本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Mmp13 ELISA Kit; Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit; UMRCASE ELISA Kit; Fragment ELISA Kit
縮寫：

MMP13
Uniprot No.：

P23097
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

93.75 pg/mL-6000 pg/mL
靈敏度：

23.44 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-90
1:2	Average %	94
	Range %	90-98
1:4	Average %	98
	Range %	93-104
1:8	Average %	102
	Range %	98-105

回收率：

The recovery of rat MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	90	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
6000	2.231	2.269	2.250	2.138
3000	1.526	1.539	1.533	1.421
1500	0.974	0.997	0.986	0.874
750	0.578	0.596	0.587	0.475
375	0.345	0.327	0.336	0.224
187.5	0.251	0.241	0.246	0.134
93.75	0.185	0.189	0.187	0.075
0	0.111	0.113	0.112

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
亞細胞定位：

Secreted, extracellular space, extracellular matrix. Secreted.
蛋白家族：

Peptidase M10A family
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000011507

UniGene: Rn.10997

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.