COL1A1 Monoclonal Antibody

說明書

中文名稱：

COL1A1鼠單克隆抗體
貨號：

CSB-MA952416
規(guī)格：

￥1090
圖片：
Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Collagen I Mouse mAb diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Human Skin Carcinoma Tissue using Collagen I Mouse mAb diluted at 1:200.
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

本產(chǎn)品為COL1A1單克隆抗體（CUSABIO貨號：CSB-MA952416），特異性識別I型膠原蛋白α1鏈（COL1A1），該蛋白是構(gòu)成細胞外基質(zhì)的主要成分，在維持組織機械強度、調(diào)控細胞黏附和信號傳導(dǎo)中發(fā)揮關(guān)鍵作用，尤其與纖維化疾病進程、腫瘤微環(huán)境重塑及骨代謝調(diào)控密切相關(guān)。該抗體經(jīng)嚴(yán)格驗證，適用于ELISA和免疫組化（IHC）實驗，可精準(zhǔn)檢測人、小鼠、大鼠等多物種樣本中COL1A1蛋白的表達定位及相對豐度，具備良好的批間穩(wěn)定性和抗原結(jié)合特異性。科研領(lǐng)域可應(yīng)用于纖維化疾病模型（如肝纖維化、肺纖維化）的機制研究、腫瘤基質(zhì)成分分析以及骨組織工程中膠原代謝的動態(tài)監(jiān)測，為探索細胞外基質(zhì)相關(guān)信號通路及病理機制提供可靠工具。該COL1A1抗體采用高純度免疫原制備，確保低交叉反應(yīng)性，適用于復(fù)雜組織樣本的多重標(biāo)記實驗，滿足分子生物學(xué)、病理學(xué)及基礎(chǔ)醫(yī)學(xué)研究的多樣化需求。
Uniprot No.：

P02452
基因名：

COL1A1
別名：

Alpha 1 type I collagen antibody; Alpha 2 type I collagen antibody; alpha 2 type I procollagen antibody; alpha 2(I) procollagen antibody; alpha 2(I)-collagen antibody; Alpha-1 type I collagen antibody; alpha1(I) procollagen antibody; CO1A1_HUMAN antibody; COL1A1 antibody; COL1A2 antibody; collagen alpha 1 chain type I antibody; Collagen alpha-1(I) chain antibody; collagen alpha-1(I) chain preproprotein antibody; Collagen I alpha 1 polypeptide antibody; Collagen I alpha 2 polypeptide antibody; collagen of skin; tendon and bone; alpha-1 chain antibody; collagen of skin; tendon and bone; alpha-2 chain antibody; Collagen type I alpha 1 antibody; Collagen type I alpha 2 antibody; EDSC antibody; OI1 antibody; OI2 antibody; OI3 antibody; OI4 antibody; pro-alpha-1 collagen type 1 antibody; type I proalpha 1 antibody; type I procollagen alpha 1 chain antibody; Type I procollagen antibody
宿主：

Mouse
反應(yīng)種屬：

Human,Mouse,Rat
免疫原：

Synthetic Peptide of Collagen I
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
純化方式：

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
克隆號：

20F10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA,IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Type I collagen is a member of group I collagen (fibrillar forming collagen).
基因功能參考文獻：
1. results strengthen the evidence for the association between polymorphisms of -1997 G/T, +1245 G/T of the COL1A1 gene in the genetic etiology of keloid scars PMID: 27511505
2. Structural basis of homo- and heterotrimerization of COL1A1/ COL1A2 has been reported. PMID: 28281531
3. patients with OI have homogenously thinner corneas compared with controls, we observed that a collagen I chain mutation was not responsible for corneal curvature alterations in OI. PMID: 30272615
4. Our findings demonstrate that collagen I could enhance the aggressive progression of residual hepatocellular carcinoma cells after suboptimal heat treatment and sorafenib may be a treatment approach to thwart this process. PMID: 30227844
5. Cellular expression of COL1A1 could promote breast cancer metastasis. COL1A1 is a new prognostic biomarker and a potential therapeutic target for breast cancer, especially in ER+ patients. PMID: 29906404
6. miR-129-5p level was decreased in fibrotic liver of human, and reduced by rOPN treatment. In contrast, miR-129-5p was induced in HSCs transfected by OPN siRNA. These data suggested that OPN induces Col 1 expression via suppression of miR-129-5p in hepatic stellate cells. PMID: 29196165
7. Depletion of MRTF-A abolished the upregulation of COL1A1 in response to the TGF-beta or Wnt signaling. PMID: 29807221
8. Mutations in the COL1A1 and COL1A2 genes associated with osteogenesis imperfecta (OI) types I or III. PMID: 29543922
9. DNMT1 was downregulated in the Lung Cancer group and its expression was further reduced in the presence of increasing malignant burden as indicated by the endobronchial findings further suggesting an Lung Cancer-specific signature. PMID: 29568927
10. our results indicated that COL1A1 promotes tumor metastasis, and that its inhibition may suppress CRC cell migration. In addition, the role of COL1A1 in CRC metastasis seems to be associated with the regulation of the WNT/PCP pathway. PMID: 29393423
11. miR378b represses the mRNA expression levels of COL1A1 via interference with SIRT6 in human dermal fibroblasts. PMID: 28983623
12. Exogenous proline stimulates type I collagen and HIF-1alpha expression and the process is attenuated by glutamine in human skin fibroblasts. PMID: 28526934
13. the efficacy of pamidronate treatment does not seem to be related to the genotype of type I collagen in patients with osteogenesis imperfecta. PMID: 28528406
14. High urine collagen is associated with renal dysfunction in lupus nephritis. PMID: 28339802
15. MiR-133a-3p could inhibit the proliferation and migration of oral squamous cell carcinoma cells through directly targeting COL1A1 and reducing its expression. PMID: 28569392
16. COL1A1 gene mutation is associated with osteogenesis imperfecta. PMID: 28810924
17. Elevation of serum alpha1(I) collagen DNA levels in scleroderma patients may be useful as the diagnostic marker, reflecting the presence of vasculopathy. PMID: 28370352
18. study showed that GG homozygotes were underrepresented in the ACL-rupture group compared with the control group, which suggests an association with reduced risk of anterior cruciate ligament injury PMID: 27632864
19. Review/Meta-analysis: possible relationship between GG genotype of COL1A1 +1245G/T polymorphism and osteoporosis risk in post-menopausal women. PMID: 28261929
20. These results support an activation mechanism of DDR1 whereby collagen induces lateral association of DDR1 dimers and phosphorylation between dimers. PMID: 28590245
21. This study identified collagen gene sets related to self-reported depression scores in healthy participants. PMID: 28334615
22. an endoplasmic reticulum complex of resident chaperones that includes HSP47, FKBP65, and BiP regulating the activity of LH2. PMID: 28177155
23. Although serum procollagen type-1 N-terminal propeptide (PINP) levels were not found to be different, tartrate-resistant acid phosphatase type 5b isoform (TRACP 5b) levels were significantly higher in the control group. PMID: 27840329
24. mutations of the COL1A1 and COL1A2 genes probably underlie the disease in the four families PMID: 28981938
25. Our results indicated that COL1A1 rs1800012 polymorphism may be associated with the reduced risk of sports-related tendon or ligament injuries, especially in ACL injuries, and that rare TT may played as a protective role. PMID: 28206959
26. Significance was detected for GG homozygous carriers (P=0.043); this genotype might be a risk factor for this type of low-density lesion (odds ratio 1.643, 95% confidence interval 1.016-2.658). PMID: 27371342
27. Breast cancer cells alter the dynamics of stromal fibronectin-collagen interactions. PMID: 27503584
28. COL1a1 crystal structure of fibronectin type III domain shows an immunoglobulin-like fold containing a beta-sandwich structure, which is formed by a three-stranded beta-sheet. PMID: 29199991
29. Alterations in the extracellular matrix microenvironment, particularly type I collagen, likely contributes to bladder cancer progression. PMID: 27655672
30. Strong correlations between the expression of type I, II, IV collagen and osteopontin and the clinical stage of tympanosclerosis indicate the involvement of these proteins in excessive fibrosis and pathological remodeling of the tympanic membrane. PMID: 29068597
31. The results support the association of COL1A1 gene polymorphisms with fracture and with low BMD at the hip in Mexican population PMID: 26423565
32. urinary N-telopeptide measured in early postmenopause is most strongly associated with rates of bone loss across the menopause transition. PMID: 27322414
33. Abnormal regulation of COL1 and COL3 may contribute to the early predisposition to POP in premenopausal women. PMID: 27636223
34. Genetic variation in COL1A1 and COL1A2 associated with osteogenesis imperfect in Vietnamese patients. PMID: 27519266
35. 2 patients with osteogenesis imperfecta (father and daughter) had a previously undescribed c.3607C>T (p.Gln1203*) change in the COL1A1 gene. PMID: 27178384
36. In the patient diagnosed with PCG and retinal detachment, analysis of WES data identified compound heterozygous variants in COL1A1 (p.Met264Leu; p.Ala1083Thr). PMID: 27484908
37. These data, together with those available in literature, suggest that vascular events are not a diagnostic handle to differentiate patients with the p.(Arg312Cys) COL1A1 mutation from those with COL5A1 and COL5A2 defects, and highlight that during the diagnostic process the presence of at least the p.(Arg312Cys) substitution in COL1A1 should be investigated in cEDS patients without type V collagen mutations. PMID: 28102596
38. we report an infant with severe OI born following a twin pregnancy in whom the bone disease is caused by a heterozygous pathogenic mutation, c.4160C >T, p.(Ala1387Val) located in the C-propeptide region of COL1A1. Our case adds to the growing evidence of the literature on mutations in the C-propeptide region being associated with a severe OI phenotype. PMID: 27549894
39. We conducted a retrospective analysis of data from clinical, laboratory, and radiographic information from children evaluated for child abuse in which molecular testing for COL1A1 and COL1A2 genes was conducted. A total of 43 patients underwent molecular testing for Osteogenesis imperfecta (OI) PMID: 27090748
40. our data revealed that COL1A1, UCP2, and PRPF40A are novel players implicated in the complex network of hypoxia response in non-small cell lung cancer PMID: 28258342
41. In the individuals with a COL1A1 mutation, 70% (7/10) of those with a glycine substitution located C-terminal of p.Gly305 exhibited DGI in both dentitions while no individual (0/7) with a mutation N-terminal of this point exhibited DGI in either dentition PMID: 28498836
42. Large COPII vesicles are transport carriers of intracellular carriers of procollagen I. PMID: 28428367
43. we established a new regulatory model of COL1A1 regulation by HIF-1, and bring out its relationship with Sp3 transcription factor. In a fundamental level, these findings give insights in the mechanisms controlling COL1A1 gene expression. PMID: 27521280
44. UBQLN4, APP, CTNNB1, SHBG, and COL1A1 might be involved in the development of nonalcoholic fatty liver disease, and are proposed as the potential markers for predicting the development of this condition PMID: 28796060
45. The levels of the bone formation marker PICP in premenopausal rheumatoid arthritis women were significantly higher than in healthy premenopausal controls. There was a highly significant difference between postmenopausal patients with RA and control pre- and postmenopausal women. Furthermore, postmenopausal RA women had significantly higher plasma PICP concentrations than premenopausal women with RA PMID: 27775453
46. Depletion of FKBP65 and inhibition of FKBP65 activity reduced the dimeric (active) form of LH2 but did not affect the binding of monomeric (inactive) LH2 to procollagen Ialpha1. PMID: 27298363
47. This is the first study to quantitatively relate pressure-induced microstructural changes in resistance arteries to the mechanics of their wall. Principal findings using a pig model system were confirmed in human arteries. The combined methods provide a strong tool for future hypothesis-driven studies of microvascular pathologies. PMID: 28432057
48. COL11A1 as a highly specific biomarker of activated cancer-associated fibroblasts in epithelial cancers. PMID: 27609069
49. miR-29b can reduce collagen biosynthesis during skin wound healing likely via post-transcriptional inhibition of HSP47 expression. PMID: 27477081
50. The study shows that circCOL3A1-859267 RNA regulate type I collagen expression in photoaged human dermal fibroblasts, suggesting it may be a novel target for interfering with photoaging. PMID: 28286269
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相關(guān)疾病：

Caffey disease (CAFFD); Ehlers-Danlos syndrome, classic type (EDS); Ehlers-Danlos syndrome 7A (EDS7A); Osteogenesis imperfecta 1 (OI1); Osteogenesis imperfecta 2 (OI2); Osteogenesis imperfecta 3 (OI3); Osteogenesis imperfecta 4 (OI4); Osteoporosis (OSTEOP)
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Fibrillar collagen family
組織特異性：

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
數(shù)據(jù)庫鏈接：

HGNC: 2197

OMIM: 114000

KEGG: hsa:1277

STRING: 9606.ENSP00000225964

UniGene: Hs.172928

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