KRT17 Monoclonal Antibody

說明書

中文名稱：

KRT17鼠單克隆抗體
貨號：

CSB-MA986157
規(guī)格：

￥1320
圖片：
IHC image of CSB-MA986157 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-MA986157 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

本產(chǎn)品為KRT17單克隆抗體（CUSABIO貨號：CSB-MA986157），專為人源KRT17蛋白檢測設(shè)計(jì)。KRT17（角蛋白17）屬于中間絲蛋白家族，在表皮細(xì)胞分化、創(chuàng)傷修復(fù)及腫瘤發(fā)生中發(fā)揮重要作用，其異常表達(dá)與多種上皮源性腫瘤及皮膚疾病密切相關(guān)。該抗體經(jīng)ELISA和免疫組化（IHC）技術(shù)驗(yàn)證，具有高特異性和批次穩(wěn)定性，適用于人類組織樣本中KRT17蛋白的定位與定量分析。科研人員可將其用于癌癥生物學(xué)研究（如鱗狀細(xì)胞癌、胰腺癌等病理機(jī)制探索）、皮膚屏障功能研究以及上皮細(xì)胞分化調(diào)控等基礎(chǔ)研究領(lǐng)域，特別適合腫瘤標(biāo)志物篩選、疾病分子分型等實(shí)驗(yàn)場景。本產(chǎn)品嚴(yán)格遵循科研級抗體質(zhì)量標(biāo)準(zhǔn)生產(chǎn)，為研究KRT17在細(xì)胞骨架重塑、腫瘤轉(zhuǎn)移等過程中的功能提供可靠工具，相關(guān)研究數(shù)據(jù)已通過多輪技術(shù)驗(yàn)證。關(guān)鍵詞：KRT17單克隆抗體、人源角蛋白17檢測、免疫組化抗體、ELISA試劑盒、腫瘤標(biāo)志物研究、細(xì)胞分化調(diào)控抗體。
產(chǎn)品名稱：

Mouse anti-Homo sapiens (Human) KRT17 Monoclonal antibody
Uniprot No.：

Q04695
基因名：

KRT17
別名：

39.1 antibody; CK 17 antibody; CK-17 antibody; Cytokeratin-17 antibody; K17 antibody; K1C17_HUMAN antibody; Keratin 17 antibody; keratin 17 epitope S1 antibody; keratin 17 epitope S2 antibody; keratin 17 epitope S4 antibody; Keratin 17, type I antibody; Keratin antibody; Keratin type I cytoskeletal 17 antibody; keratin, type i cytoskeletal 17 [version 1] antibody; Keratin-17 antibody; KRT17 antibody; PC antibody; PC2 antibody; PCHC1 antibody; type I cytoskeletal 17 antibody
宿主：

Mouse
反應(yīng)種屬：

Human
免疫原：

Synthesized peptide derived from human Cytokeratin 17
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG1, Kappa
純化方式：

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
克隆號：

11C5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors. May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.
基因功能參考文獻(xiàn)：
1. A report of a recurrent mutation in the KRT17 gene found in a family with severe SM, which provides further evidence that some factors such as modifiers, androgenic stimulation and/or environment influence the phenotypes, and other genetic or proteomic conditions might also influence the final manifestations of the disease. PMID: 29218738
2. High CK17 expression is associated with lymph node metastasis in oral squamous cell carcinoma. PMID: 30066921
3. High expression of KRT17 is frequent in Lung adenocarcinoma tissues. PMID: 29991674
4. Narrow-band ultraviolet B irradiation could downregulate K17 expression by inhibiting the ERK1/2 and STAT3 signaling pathways. PMID: 29349514
5. Our results highlight KRT17 as a possible biomarker in gastric cancer promoting tumor growth, motility, and invasion, and suggest that KRT17 can be a valuable molecular target for development of anti-gastric cancer-specific therapies PMID: 28299464
6. The findings suggest that CK17, although not specific for differentiated vulvar intraepithelial neoplasia (dVIN), when combined with histologic findings, Ki-67, and p53 immunohistochemistry, can be a marker of vulvar dysplasia and serve as an adjunct in the diagnosis of dVIN. PMID: 27513074
7. Krt17 was demonstrated to contribute to areca nut-induced oral malignancy PMID: 27432155
8. TGFbeta/SMAD/miR-486-3p signaling axis in keratinocytes regulated K17 expression and cell proliferation. We conclude that the loss of miR-486-3p in psoriatic epidermis leads to K17 protein overexpression and contributes to the pathogenesis of psoriasis. PMID: 28642156
9. KRT17 promotes tumor cell growth, at least partially, through its anti-apoptotic effect as a result of the KRT17 overexpression by GLIs in oral squamous cell carcinoma (OSCC).. PMID: 28342001
10. TGF-beta1-induced CK17 enhances cancer stem cell-like properties rather than epithelial-mesenchymal transition in promoting cervical cancer metastasis via the ERK1/2-MZF1 signaling pathway. PMID: 28703907
11. K17 is highly expressed in most cases of both invasive adenocarcinoma and in AIS and is a powerful, negative prognostic marker for patient survival. PMID: 28821199
12. the data suggest that the loss of Krt17 may foster an overall protective environment for lesion-prone cervical tissue. PMID: 27065324
13. High KR17 expression is associated with oral cancer. PMID: 27512993
14. K17 is expressed in a subset of triple-negative breast cancers, and is a marker of poor prognosis in patients with advanced stage and ER(-)/HER2(-) breast cancer. PMID: 27816721
15. MiR-138 may regulate K17 protein expression to affect cell proliferation and apoptosis by targeting hTERT gene in keratinocytes from psoriatic vulgaris patients. PMID: 27936398
16. The pattern of CK17 expression is a highly sensitive marker for distinguishing cutaneous lymphadenoma from basal cell carcinoma in small samples. PMID: 26968559
17. Cytokeratin 17 immunoexpression might have a role in evaluating surgical borders in some cases of actinic keratosis and Bowen disease. PMID: 26740287
18. Steatocystoma multiplex is associated with the R94C mutation in the KRTl7 gene, and is reported in a Chinese pedigree. PMID: 26165312
19. These findings suggest that CK17 is involved in the development and metastasis of papillary thyroid carcinoma PMID: 26191283
20. Case Report: polycystic kidney disease with steatocystoma multiplex. PKD1 mutations disrupt keratin 17 polymerization. PMID: 25111597
21. our findings establish that K17 functions specially among keratins as an oncoprotein by controlling the ability of p27(KIP1) to influence cervical cancer pathogenesis. PMID: 26109559
22. Data indicate that autoimmune regulator Aire mRNA transcripts are regulated in a keratin 17 (K17) dependent manner in skin tumor keratinocytes. PMID: 26168014
23. We conclude that K17 expression promotes oral squamous cell carcinoma cell growth and cell size but does not affect cell migration PMID: 25736868
24. Report overexpression of keratin 17 in premalignant and malignant squamous lesions of the cervix. PMID: 24051697
25. novel heterozygous mutation, p.L91P (c.272T>C) in the helix initiation motif, associated with pachyonychia congenita type 2 PMID: 23855588
26. Peripheral or diffuse staining for CK17 is a useful marker of invasion in anal squamous neoplastic lesions. PMID: 24335642
27. KRT17 is necessary for oncogenic transformation in Ewing sarcoma and accounts for much of the GLI1-mediated transformation function but via a mechanism independent of AKT signaling. PMID: 24043308
28. Overexpression of keratin 17 is associated with epithelial ovarian cancer. PMID: 23430585
29. a novel mutation in a Chinese pedigree of pachyonychia congenita type 2 with typical clinical presentations and an autosomal dominant inheritance pattern PMID: 23278621
30. We identified a known mutation in the KRT17 gene in a family with steatocystoma PMID: 22639854
31. Keratin-17 expression is correlated with tumor progression in gastric adenocarcinoma and may serve as a biomarker for poor prognosis. PMID: 22695933
32. IL-22 up-regulates K17 expression in keratinocytes in a dose-dependent manner through STAT3- and ERK1/2-dependent mechanisms. PMID: 22808266
33. Keratin 17 is a therapeutic target for the treatment of psoriasis [review] PMID: 22795618
34. We observed a higher likelihood of oral leukokeratosis in individuals harboring KRT6A mutations, and a strong association of natal teeth and cysts in carriers of a KRT17 mutation. PMID: 22264670
35. Data indicate that cytokeratin 17 (CK17) expression could be associated with the differentiation and the malignancy of oral squamous cell carcinoma (OSCC). PMID: 22466643
36. A report on homozygosity for dominant missense mutations in keratin 17 that modify the pachyonychia congenita phenotype. PMID: 22336949
37. Both moesin and KRT17 demonstrated a tendency of increased expression as pT stage advanced. PMID: 22076435
38. a novel interaction involving K17 and AnxA2 and identify AnxA2 as a potential regulator of keratin filaments. PMID: 22235123
39. Type I keratin 17 protein is phosphorylated on serine 44 by p90 ribosomal protein S6 kinase 1 (RSK1) in a growth- and stress-dependent fashion PMID: 22006917
40. The data suggested that IL-17A can upregulate keratin-17 expression in keratinocytes in a dose-dependent manner through STAT1- and STAT3-dependent mechanisms. PMID: 21796151
41. AIRE expression in HaCaT epidermal keratinocytes, as well as its interaction with K17, was confirmed. PMID: 21356351
42. KRT17 is upregulated in gastric adenocarcinoma and is associated with tumor progression. PMID: 21443102
43. mutation of KRT17 may play a major role in the pathogenesis of this pedigree with pachyonychia congenita type 2. PMID: 21287500
44. Overexpression of KRT17 is associated with basal-like phenotype in breast cancer. PMID: 19882246
45. CK17 expression is significantly higher in thyroid papillary carcinoma than benign thyroid tissue; and this characteristic can have important diagnostic value. PMID: 14761598
46. Mutation in second half of 1A domain of K17 might delay onset of clinical phenotype. PMID: 15102078
47. Epitopes S1 (118-132), S2 (169-183), S4 (323-337) and S4 (348-362) are immunodominant DR B1-restricted T cell epitopes for psoriasis. S1 (118-132) contains the ALEEAN sequence. Others with different amino acid sequence have not been reported before. PMID: 15795121
48. KRT 17 seemed to be the most accurate marker for the diagnosis of micrometastases of a size >450 mum. PMID: 16638858
49. findings showed that out of all cytokeratins, CK17 was up-regulated strongest in oral squamous cell carcinoma compared to normal samples, and over-expression was most significantly associated with diagnosis PMID: 17786476
50. The mutation p.M88K is in the helix initiation motif (HIM) of KRT17, where 13 of the reported 14 mutations of KRT17 in PC-2 have been mapped, and the residue M88 of the HIM is also a mutation hotspot of other keratin disorders (http://www.interfil.org/). PMID: 18547302
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相關(guān)疾病：

Pachyonychia congenita 2 (PC2); Steatocystoma multiplex (SM)
亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Intermediate filament family
組織特異性：

Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epider
數(shù)據(jù)庫鏈接：

HGNC: 6427

OMIM: 148069

KEGG: hsa:3872

STRING: 9606.ENSP00000308452

UniGene: Hs.2785

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