Recombinant Human Cytokine receptor common subunit beta (CSF2RB), partial (Active)

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品名簡(jiǎn)稱(chēng)：

Recombinant Human CSF2RB protein, partial (Active)
貨號(hào)：

CSB-MP006047HU
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：

￥696
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized CSF2RB at 2 μg/ml can bind anti-CSF2RB antibody（CSB-RA006047A0HU）, the EC₅₀ is 0.1594-0.3046 ng/mL.
其他：

產(chǎn)品詳情
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靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
內(nèi)毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized CSF2RB at 2 μg/mL can bind anti-CSF2RB antibody（CSB-RA006047A0HU）, the EC₅₀ is 0.1594-0.3046 ng/mL.
基因名：

CSF2RB
Uniprot No.：

P32927
別名：

(CDw131)(GM-CSF/IL-3/IL-5 receptor common beta subunit)(CD antigen CD131)
分子結(jié)構(gòu)：
種屬：

Homo sapiens (Human)
蛋白長(zhǎng)度：

Partial
來(lái)源：

Mammalian cell
分子量：

50.9 kDa
表達(dá)區(qū)域：

17-443aa
氨基酸序列

WERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPATHGQYIVSVQPRRAEKHIKSSVNIQMAPPSLNVTKDGDSYSLRWETMKMRYEHIDHTFEIQYRKDTATWKDSKTETLQNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEARSWDTESVLPMW
蛋白標(biāo)簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

This Human CSF2RB recombinant protein was produced in mammalian cell, where the gene sequence encoding Human CSF2RB (17-443aa) was expressed with the C-terminal 6xHis tag. The purity of this CSF2RB protein was greater than 95% by SDS-PAGE. The activity has been validated.
As a β subunit shared by three cytokine receptors of IL-3, GM-CSF and IL-5, CSF2RB mainly plays the role of signaling cytokines. There are many studies on hematological diseases. CSF2RB promotes both proliferation and differentiation of hematopoietic cells, and plays an important role in maintaining the balance of proliferation, differentiation and self-renewal of hematopoietic cells. Further research on the structure and function of CSF2RB is conducive to the exploration of the malignant proliferation mechanism of leukemia cells, and lays a foundation for further research and treatment strategies for leukemia from the receptor/ligand molecular level. CSF2RB protein can be used as a specific marker protein for the diagnosis of prostate cancer bone metastasis, which makes the diagnosis of prostate cancer bone metastasis more accurate and rapid. The CSF2RB protein can also be used as a molecular drug for the preparation of prostate cancer bone metastasis, and provides a new therapeutic approach for prostate cancer bone metastasis.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

平均分:

5.0分 - 1 個(gè)評(píng)價(jià)

實(shí)驗(yàn)應(yīng)用：藥物相關(guān)研究

產(chǎn)品評(píng)價(jià)：收到產(chǎn)品后，我們進(jìn)行ELISA實(shí)驗(yàn)，結(jié)果顯示活性好，后續(xù)用于藥物高通量篩選，實(shí)驗(yàn)進(jìn)展順利，蛋白比較穩(wěn)定，重復(fù)性好！

By 王老師

靶點(diǎn)詳情

功能：

High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
基因功能參考文獻(xiàn)：
1. SYK mediates the actions of EPO and GM-CSF and coordinates with TGF-beta in erythropoiesis. PMID: 28131718
2. Exome sequencing identified candidate variants, including a missense mutation in DUOX2 that impaired its function and a frameshift mutation in CSF2RB that was associated with Crohn's Disease in an independent cohort of Ashkenazi Jewish individuals. PMID: 27373512
3. In a genetic analysis of Ashkenazi Jewish individuals, we associated Crohn's Disease with a frameshift mutation in CSF2RB. Intestinal monocytes from carriers of this mutation had reduced responses to granulocyte-macrophage colony-stimulating factor, providing an additional mechanism for alterations to the innate immune response in individuals with Crohn's Disease. PMID: 27377463
4. VitD-mediated stimulation of GC anti-inflammatory affects human monocytes in a process involving GM-CSF and MED14 PMID: 23572530
5. These data suggest that expression of GM-CSF and its receptor subunits by colon tumors may be a useful marker for prognosis PMID: 23108143
6. JAK kinase binding to betac requires the presence of three critical betac lysine residues, and this binding event is essential for receptor ubiquitination, endocytosis, and signaling. PMID: 21965659
7. Over expression of IL-3Ralpha and truncated mutation of hbetac may be involved in proliferation and differentiation block in NB4 cells. PMID: 21176354
8. Granulocyte/macrophage colony-stimulating factor causes a paradoxical increase in the BH3-only pro-apoptotic protein Bim in human neutrophils PMID: 20705940
9. The results of the study support CSF2RB as a risk factor common to both schizophrenia and major depression in the Chinese Han population. PMID: 21247258
10. Hereditary pulmonary alveolar proteinosis caused by recessive CSF2RB mutations [case report] PMID: 21205713
11. the up-regulation of IL-3Rbeta expression may contribute to the maintenance of proliferation rather than cell differentiation. PMID: 21207215
12. The beta1 integrin-interacting domain in the extracellular domain of IL-3Rbeta was identified. A fragment of a protein corresponding to domain 4 of IL-3Rbeta prevented IL-3-mediated arterial morphogenesis and endothelial cell migration. PMID: 20802515
13. the domain 1 D-E loop disulfide of hbetac and beta(IL-3) have roles in maintaining the precise positions of ligand-binding residues necessary for normal high affinity binding and signaling PMID: 20516062
14. Two different modes of beta c binding are utilized in the presence of the hIL-3R alpha isoforms. PMID: 20472554
15. hGM-CSF activation of hGM/beta(c)with or w/o the hbeta(c) subunit, promoted primitive phenotype maintenance, indicating that the hGM Ralpha chain cytosolic domain is needed for differentiation mediated by the hGM Ralpha, beta(c) receptor complex. PMID: 12384414
16. JAK2 activation mediates cross-talk between extracellular domain mutants of the beta-subunit of the GM-CSF receptor and EpoR PMID: 12488507
17. Interleukin-3 binding to the human betac receptor involves functional epitopes formed by domains 1 and 4 of different protein chains PMID: 15060062
18. A minor improvement in resolution has given a clearer indication of the position and stabilization of the key residues Tyr15, Phe79, Tyr347, His349, Ile350 and Tyr403 in the elbow region between domain 1 and domain 4 of the dimer-related molecule. PMID: 16754968
19. These results suggest that the JAK1-JH7-3 domains are required for interleukin-3 receptor common beta subunit interaction and abolish wild type JAK1 and JAK2-mediated signaling. PMID: 16767694
20. This study found that several markers (rs2072707, rs2284031 and rs909486)of csf2rb showed sex-specific and family history-dependent associations with schizophrenia. PMID: 17667962
21. The dynamic role of the common signaling subunit beta c is to prevent IL5alpha and GM-CSF ligand-receptor complexes from rapidly dissociating. PMID: 18294864
22. The interaction between intercellular adhesion molecule 1 (ICAM-1) and the GM-CSF receptor is essential for GM-CSF-induced eosinophil activation and survival. PMID: 18322230
23. IL-5-induced effects on betac assembly in the presence of nontagged IL-5Ralpha provide direct evidence that IL-5 can cause higher order rearrangements of betac homo-oligomers PMID: 18326494
24. Ligand-binding and receptor activation are not critically dependent on individual N-glycosylation sites within the IL-3- and IL-5-receptor common beta-subunit. PMID: 18374598
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相關(guān)疾病：

Pulmonary surfactant metabolism dysfunction 5 (SMDP5)
亞細(xì)胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Type I cytokine receptor family, Type 4 subfamily
數(shù)據(jù)庫(kù)鏈接：

HGNC: 2436

OMIM: 138981

KEGG: hsa:1439

STRING: 9606.ENSP00000384053

UniGene: Hs.592192