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中文名稱：BMP4重組抗體
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貨號：CSB-RA257673A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: BMP4 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 36-55 kDa -
Immunofluorescence staining of Hela cell with CSB-RA257673A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 508-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產品詳情
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產品描述：CSB-RA257673A0HU BMP4重組單克隆抗體是針對骨形態發生蛋白4（BMP4）研發的高特異性檢測工具，該靶標屬于TGF-β超家族成員，在胚胎發育、組織穩態及細胞命運決定中發揮關鍵調控作用。本產品經過ELISA、蛋白質印跡（WB）和免疫熒光（IF）多平臺嚴格驗證，可在多種樣本中精準識別內源性BMP4蛋白，其中WB實驗顯示清晰單一的目標條帶，推薦使用稀釋比例為1:500-1:2000；IF實驗在1:50-1:200稀釋范圍內呈現顯著的特異性信號定位。適用于研究BMP4在干細胞定向分化、骨骼肌肉發育、腫瘤微環境調控及再生醫學等領域的分子機制。抗體為即用型液體制劑（濃度1mg/mL），含穩定劑可長期保存于-20°C，其重組單克隆特性確保批次間高度一致性，為體外細胞實驗、組織樣本分析等基礎科研提供可靠解決方案。
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Uniprot No.：
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基因名：
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別名：Bone morphogenetic protein 4 (BMP-4) (Bone morphogenetic protein 2B) (BMP-2B), BMP4, BMP2B DVR4
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反應種屬：Human
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免疫原：A synthesized peptide derived from human BMP4
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：19B7
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IF 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻
產品評價
相關產品
靶點詳情
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功能：Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical BMP pathways such as ERK/MAP kinase, PI3K/Akt, or SRC cascades. For example, induces SRC phosphorylation which, in turn, activates VEGFR2, leading to an angiogenic response.
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基因功能參考文獻：
- The expression of BMP4 and FGF8 corelates well with the proliferative component of the pathologies, indicating a possible role in the pathogenesis and progression of Odontogenic Cyst and Tumors. PMID: 30079292
- We detected a haplotype-based interaction for BMP4 and IRF6 genes for expression of the orofacial cleft phenotype. Although the MDR methods indicate that the effect of interaction between these genes appears to be mild, the presence of specific haplotype combinations conferring a higher risk for NSCL/P reveals the possible combined role of these genes in the pathogenesis of this prevalent birth defect. PMID: 28133786
- Data show that bone morphogenetic protein 4 (BMP4) expression is associated and favored type II macrophage differentiation. PMID: 28928159
- MiR-876-5p suppresses epithelial-mesenchymal transition of lung cancer by directly down-regulating BMP-4. PMID: 29229885
- endothelial BMP4 controls leukocyte recruitment through a VE-cadherin-dependent mechanism and BMP4-induced inflammation might be involved in the pathogenesis of endothelial cell damage following successful resuscitation after cardiac arrest. PMID: 28755278
- Studied serum levels of bone morphogenic protein-4 (BMP-4) and matrix Gla protein (MGP) in patients who were admitted to emergency department with the diagnosis of acute coronary syndrome (ACS) and underwent primary percutaneous coronary intervention. MGP and BMP-4 levels were significantly elevated when compared to subjects with normal coronary arteries. PMID: 28605143
- BMP4 inhibits epithelial-mesenchymal transition of the retinal pigment epithelium. PMID: 27586653
- Potentially functional and tagging SNPs of BMP2 (rs170986, rs1979855, rs1980499, rs235768, rs3178250) and BMP4 (rs17563, rs4898820, rs762642) were genotyped in NSCLC. PMID: 28574846
- BMP4 polymorphic site rs4901474 (T>C) had an effect on hypertension; CC genotype carriers had a 1.48-fold risk for hypertension at the age of 50 years when compared with T-allele carriers PMID: 29390526
- High serum BMP4 levels are associated with postmenopausal osteoporosis. PMID: 28238166
- results indicate that the BMP4 rs17563 variant is likely to confer a protective effect against the occurrence of Non-Syndromic Cleft Lip with or without Cleft Palate in a sample of the southeast Iranian population. PMID: 29211286
- cardiomyocyte (CM) conditioned medium can trigger the recruitment of pro-inflammatory (M1) macrophages, through a mechanism that involves, in part, CM-derived BMP4. PMID: 26103914
- BMP4 participates in the regulation of invasion and migration by EC109/Taxol cells PMID: 28315766
- Bone morphogenetic protein 4 regulates expression of microRNAs miR-494 and miR-126-5p to control endothelial cell function in angiogenesis. PMID: 28124060
- BMP4 expression was significantly increased in HCC tissue, and was correlated with tumor de-differentiation and unfavorable prognosis. BMP4 promoted HCC EMT and was correlated with OXA resistance. PMID: 28987388
- Gene expression profiling showed that MuSK was required for the BMP4-induced expression of a subset of genes in myoblasts, including regulator of G protein signaling 4 (Rgs4). PMID: 27601729
- BMP4 levels are increased dramatically in individuals with impaired glucose tolerance and type 2 diabetes. PMID: 27524617
- The results suggest that selective RNA decay via TGF-beta and BMP4 signaling is critical for specifying the developmental fate of specific human embryonic cell lineages. PMID: 27304915
- Expression of bone morphogenetic protein (BMP) 4, an upstream stimulator of SOX9, was upregulated by CG. PMID: 27931264
- Study shows that BMP4 is overexpressed in hepatocellular carcinoma (HCC) tissues and BMP4-induced ID2/CDKN1B signaling facilitates the proliferation of HCC. PMID: 28543546
- we demonstrate that expression of bone morphogenetic protein 4 (BMP-4) is universally upregulated in human colorectal cancer cells and tissues, resulting in activated BMP signaling PMID: 28611046
- RUNX1T1 serves as a common angiogenic driver for vaculogenesis and functionality of endothelial lineage cells PMID: 28640846
- Phosphorylated Smad1/5/8/9 specifically bound to the BREs of Smad8/9 gene. The present study reveals that Smad8/9 is a unique R-Smad regulated through the BMP pathway at the mRNA level. PMID: 26748560
- Meta-analysis to assess the effect of BMP4 rs17563 polymorphism on nonsyndromic cleft lip with or without cleft palate (NSCL/P) suggests that the C allele of BMP4 rs17563 may be a risk factor for NSCL/P among Asians and Caucasians, and may be a protective factor for NSCL/P in Brazilian population. PMID: 28767592
- this study identified BMP-4 as a potential molecular marker for predicting the invasion and progression of papillary thyroid carcinoma PMID: 28214211
- Bmp4 drives terminal differentiation into mature white rather than brown fat cells. Bmp4 seems to have a dual role in metabolism either promoting or repressing oxidative metabolism in a cell context dependent manner. PMID: 28425843
- Data suggest that serum BMP4 levels are associated with visceral adiposity and may play role in fat distribution; role of BMP4 in males and females may be different; visceral adiposity may predict serum BMP4 levels in females with obesity; serum BMP4 levels are decreased after GLP-1 receptor agonist (exenatide) treatment in obesity. This study was conducted in China. PMID: 28376799
- BMP4 is a potential cause of digital and eye abnormalities in familial dopa-responsive dystonia. PMID: 28558098
- BMP4 expression was significantly upregulated in esophageal adenocarcinoma and Barrett's esophagus. BMP4 incubation inhibited cell viability,induced cell migration adnd upregulated SNAIL2 expression. PMID: 27191723
- the data identify MxA as a novel stimulator of BMP4 and BMP9 transcriptional signaling, and suggest it to be a candidate IFN-alpha-inducible mechanism that might have a protective role against development of pulmonary arterial hypertension and other vascular diseases. PMID: 27875556
- The 6007C>T polymorphism of BMP-4, -66T>G polymorphism of OPN, and VDR-FokI polymorphism are the susceptible factors of spinal TB and indicators of the clinical severity. These three genes may collaborate in the development of spinal TB. PMID: 28376475
- The results suggest that Osterix plays an important role in increasing BMP- 4-induced Cx43 activity. PMID: 27498006
- inhibitory role on glucagon secretion, pancreatic alpha-cell growth, and expression of genes maintaining alpha-cell identity PMID: 27479530
- BMP4 promotes a phenotype change of an esophageal squamous epithelium via up-regulation of KLF4. PMID: 27693253
- Study demonstrated that Chrdl1 acts as an inhibitor of BMP4-induced migration and invasion which stimulated breast cancer cell invasion and matrix degradation, in part, through MMP2 and MMP9 activity that is antagonized by Chrdl1. PMID: 26976638
- BMP4 GAAA (p=0.05) and GGGA (p=0.02) haplotypes were associated with peri-implantitis (p=0.03). Therefore, it may be concluded that BMP4 and FGF10 haplotypes are associated with peri-implantitis PMID: 27652695
- BMP4 inhibited ERalpha signaling by increasing the degradation of ERalpha, and that the expression of BMP4 itself was suppressed by estrogens. PMID: 27522322
- study shows that patients with CRA had high expression of BMP6 and hepcidin and low expression of s-HJV. BMP6 was found to be negatively correlated with s-HJV; both regulate hepcidin expression and play important roles in the development of anemia. PMID: 27051019
- we have for the first time characterized the BMP4-induced miRNA expression profiles in breast cancer cell lines, showing that induced miRNAs contribute to the fine-tuning of proliferation and migration phenotypes. PMID: 26684238
- BMP4 caused a trend towards accelerated metastasis formation, especially in bone. More work is needed to uncover the long-term effects of BMP4 and the clinical relevance of these findings. PMID: 26970275
- BMP4 is a direct thyroid hormone target and is involved in a positive autoregulatory feedback loop that modulates thyroid hormone signaling PMID: 26676745
- An up-regulated expression of BMP-4 and BMPR-II was observed in the peripheral blood of breast cancer patients especially in the advanced-stage of the disease. Moreover, BMP-4 and BMPR-II expressions were found to be correlated PMID: 26406943
- PDGF-AA impairs endothelium-dependent vasodilation and PDGF-AA mediates BMP4-induced adverse effect on endothelial cell function through SMAD1/5- and SMAD4-dependent mechanisms. PMID: 26769046
- BMP4 gene is involved in the ATO regulation of adipogenic and osteogenic differentiation balance, which provides a new target for the treatment of AA PMID: 26215597
- Overexpression of the BMP4/SMAD4/SMAD5 signaling pathway could predict poor clinical outcome in skull base chordomas, suggesting activation of this pathway is involved in chordoma pathogenesis. PMID: 26339396
- HIF-1alpha knockdown attenuated hypoxia-induced BMP4 expression and knockdown of either HIF-1alpha or BMP4 abolished hypoxia-induced TRPC expression and basal [Ca(2+)]i. PMID: 25824146
- The effect of TGF-beta1 and BMP-4 on bone marrow-derived stem cell morphology on a novel bioabsorbable nanocomposite material. PMID: 24245787
- Suggest that hypermethylation of ACP1, BMP4, and TSPYL5 are common events in HCC and could be used as potentially detectable biomarkers in HCC tissues. PMID: 26386860
- Additive Effects of Retinoic Acid (RA) and Bone Morphogenetic Protein 4 (BMP-4) Apoptosis Signaling in Retinoblastoma Cell Lines PMID: 26173116
- findings support a role of the BMP4 gene in the aetiology of non-syndromic CL/CLP and CP in the Latvian and Lithuanian populations. PMID: 25471993
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相關疾病：Microphthalmia, syndromic, 6 (MCOPS6); Non-syndromic orofacial cleft 11 (OFC11)
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亞細胞定位：Secreted, extracellular space, extracellular matrix.
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蛋白家族：TGF-beta family
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組織特異性：Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
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