DSG3 Recombinant Monoclonal Antibody

說明書

中文名稱：

DSG3重組抗體
貨號(hào)：

CSB-RA007205A0HU
規(guī)格：

￥1320
圖片：
The Binding Activity of Human DSG3 with Anti-DSG3 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized human DSG3 (CSB-MP007205HUc7) at 2 μg/mL can bind Anti-DSG3 recombinant antibody, the EC₅₀ is 10.16-12.87 ng/mL.

The Binding Activity of Mouse Dsg3 with Anti-DSG3 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Dsg3 (CSB-MP007205MOd7) at 5 μg/mL can bind Anti-DSG3 recombinant antibody, the EC₅₀ is 2.624-3.182 μg/mL.

The Binding Activity of Human DSG3 with Anti-DSG3 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human DSG3 (CSB-BP007205HUc7) at 2 μg/mL can bind Anti-DSG3 recombinant antibody, the EC₅₀ is 4.552-5.720 ng/mL.

The Binding Activity of Human DSG3 with Anti-DSG3 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized human DSG3 (CSB-MP007205HUc7) at 2 μg/mL can bind Anti-DSG3 recombinant antibody. The EC₅₀ is 3.915-4.410 ng/mL.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

DSG3重組單克隆抗體（CSB-RA007205A0HU）是一款針對(duì)橋粒芯糖蛋白3（Desmoglein 3, DSG3）開發(fā)的高特異性科研工具。DSG3作為鈣黏蛋白家族成員，廣泛表達(dá)于上皮細(xì)胞的橋粒結(jié)構(gòu)中，通過介導(dǎo)細(xì)胞間黏附參與維持皮膚和黏膜屏障的完整性，其功能異常與天皰瘡等自身免疫性皮膚疾病密切相關(guān)。本抗體采用重組單克隆技術(shù)制備，經(jīng)過嚴(yán)格的ELISA驗(yàn)證，展現(xiàn)出優(yōu)異的抗原結(jié)合能力與批次穩(wěn)定性，可精準(zhǔn)識(shí)別天然及重組DSG3蛋白。該產(chǎn)品適用于體外實(shí)驗(yàn)中對(duì)DSG3蛋白表達(dá)的定性或定量檢測(cè)，可支持自身免疫性水皰病機(jī)制研究、上皮細(xì)胞黏附功能分析以及相關(guān)分子信號(hào)通路探索等科研場(chǎng)景，為病理模型構(gòu)建、候選藥物靶點(diǎn)驗(yàn)證及基礎(chǔ)醫(yī)學(xué)研究提供可靠工具。本產(chǎn)品嚴(yán)格限定于科學(xué)研究用途。
Uniprot No.：

P32926
基因名：

DSG3
別名：

130 kD pemphigus vulgaris antigen; 130 kDa pemphigus vulgaris antigen; Balding; Cadherin family member 6; CDHF 6; CDHF6; Desmoglein 3; Desmoglein 3 precursor; Desmoglein-3; Desmoglein3; DKFZp686P23184; DSG 3; DSG3; DSG3_HUMAN; Pemphigus vulgaris antigen; PVA
反應(yīng)種屬：

Human, Mouse
免疫原：

Recombinant Human DSG3 protein
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

mIgG1
純化方式：

Affinity-chromatography
克隆號(hào)：

4C2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA
Protocols：

ELISA Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
基因功能參考文獻(xiàn)：
1. flotillin has a role in desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes PMID: 27346727
2. our findings suggest that Dsg3 functions as a key in cell-cell adhesion through at least a mechanism of regulating E-cadherin membrane trafficking PMID: 27254775
3. Desmoglein 3 Order and Dynamics in Desmosomes PMID: 29212005
4. Data indicate that all 3 monoclonal antibodies (mAbs) targeted the same extracellular cadherin (EC) domain on desmoglein 3 (Dsg3), caused Dsg3 internalization in primary keratinocytes, and caused suprabasal blisters in skin. PMID: 27304671
5. DSG3 was a key prognostic factor and predictor for neoadjuvant concurrent chemoradiotherapy response in rectal cancer patients. PMID: 27040321
6. Treg cells exert a clear down-regulatory effect on the Dsg3-driven T-cell response and, accordingly, the formation of Dsg3-specific IgG antibodies PMID: 26661498
7. Data identify DSG3 as a negative prognostic biomarker in resected pancreatic ductal adenocarcinoma, as high DSG3 expression is associated with poor overall survival and poor tumour-specific survival. PMID: 26469831
8. this study directly demonstrates the occurrence of desmoglein 3 molecules outside of desmosomes and reveales that the adhesive properties of these molecules do differ depending on their localization. PMID: 25510735
9. DSG2 and DSG3 might be potential diagnostic markers for squamous cell carcinoma of the lung and DSG3 could be a potential differentiation marker for lung cancer. PMID: 25468811
10. alterations of their expression suggest a role of Dsg3 and gamma-catenin (additionally to E-cadherin/beta-catenin) as biomarkers of malignant transformation risk of oral dysplasia and the biological behavior (aggressiveness) of oral cancer, respectively PMID: 23430339
11. the Dsg3 epitopes targeted by pathogenic mPV IgG are human specific in mice PMID: 25695683
12. Pemphigus vulgaris is characterized by low IgG reactivities to specific self-antigens along with high IgG reactivity to desmoglein 3. PMID: 24820664
13. High DSG3 mRNA expression is associated with esophageal squamous cell carcinoma. PMID: 24568510
14. Pemphigus vulgaris autoimmune globulin induces Src-dependent tyrosine-phosphorylation of PKP3 and its detachment from DSG3. PMID: 24328683
15. Dsg3 did not associate with rafts in cells lacking desmosomal proteins. PMID: 24498201
16. this study identifies a novel Dsg3-mediated c-Jun/AP-1 regulatory mechanism and PKC-dependent Ezrin phosphorylation that could be responsible for Dsg3-associated cancer metastasis. PMID: 23752190
17. DSG3 functions as an oncogene and facilitates cancer growth and invasion in head and neck cancer cells PMID: 23737966
18. The conformational Dsg3 ELISA index reflected the pathogenicity of anti-Dsg3 antibodies more accurately than the conventional Dsg3 ELISA index in pemphigus vulgaris. PMID: 23602628
19. MAC387 and desmoglein-3 are reliable diagnostic markers for supporting the morphologic impression of squamous differentiation in urinary bladder carcinoma. PMID: 23806524
20. All 3 markers were studied independently and were associated with tumor percentage in metastatic lymph nodes. PVA had the strongest correlation, followed by PTHrP and then TACSTD1. PMID: 23625795
21. provide a proof of principle supporting that ultrasensitive nanostructured assay systems for DSG3 can be exploited to detect micrometastatic HNSCC lesions in lymph nodes, which can improve the diagnosis PMID: 23010602
22. The cis-adhesive interface on extracellular subdomain EC1 recognized by the pathogenic antibody PVA224 is the primary target of the autoantibodies present in the serum of pemiphigus vulgaris patients. PMID: 22996451
23. an important novel function for Dsg3 in promoting actin dynamics through regulating Rac1 and Cdc42 activation in epithelial cells. PMID: 22796473
24. a useful immunohistochemical marker for differentiation of lung squamous cell carcinoma and adenocarcinoma from other subtypes PMID: 22495379
25. In paraneoplastic pempigus and pemphigus vulgaris, IgG autoantibodies to DSG3 may be found in autoantibody profiling. PMID: 22318391
26. Report mapping of B cell epitopes on desmoglein 3 in pemphigus vulgaris patients by the use of overlapping peptides. PMID: 22261006
27. These findings suggest a novel function for Dsg3 in the control of E-cadherin-Src signalling and cell-cell adhesion. PMID: 22294297
28. These data indicate a contribution of Dsg depletion to pemphigus vulgaris pathogenesis dependent on Ca(2+)-induced differentiation. PMID: 21864491
29. Silencing desmoglein 3 caused defects in cell-cell adhesion and concomitant reduction in cell proliferation in both HaCaT and MDCK cells. PMID: 21702856
30. Dsg3, as an up-stream regulator of Src activity, helps regulate adherens junction formation through its interaction with E-cadherin PMID: 21151980
31. The genetic background of the local population may explain why pemphigus occurs more commonly than bullous pemphigoid in Northwestern Romania compared with the population of Western Europe PMID: 20618495
32. results argue against the hypothesis that DSG3 coding variants play a role in pemphigus vulgaris susceptibility PMID: 19678820
33. Data strongly support the view that desmoglein 3 contributes to the regulation of epidermal differentiation. PMID: 12138195
34. The appearance of DSG3-reactive Th2 cells is constant at different stages of pemphigus vulgaris (PV), while DSG3-reactive Th1 cells are detected at a significantly higher frequency in chronic active PV. PMID: 12496453
35. In trichilemmal keratinization in follicle, and in cysts in these areas, desmoglein 3 expressed throughout outer root sheath and cyst wall. In areas of epidermal-like keratinization, desmoglein 3 expression was limited mainly to the basal layer. PMID: 12787134
36. a novel negative marker for epidermal stem cell-containing population of keratinocytes PMID: 12953062
37. Dsg3(AA145-192)-specific cells preferentially utilize the TCRVbeta13 gene, while Dsg3(AA240-303)- and Dsg3 (AA570-614)-specific cells utilize Vbeta7 and Vbeta17 genes, respectively PMID: 14675184
38. The five genes, and three of the encoded proteins, were shown differentially expressed between a group of keratoconus patients and a reference group using different techniques PMID: 16015083
39. Dsg3 endocytosis, keratin filament retraction, and the loss of keratinocyte cell-cell adhesion are coordinated responses to PV IgG PMID: 16377623
40. autoantibodies against desmoglein 3 (Dsg3) have been detected in sera from patients with fogo selvagem PMID: 16763546
41. analysis of desmoglein 3 ectodomain in pemphigus vulgaris PMID: 16842599
42. Overexpression of DSG3 is associated with head and neck cancer PMID: 16878157
43. Desmoglein 3 status indicated a poor prognosis in lung cancers. PMID: 17084439
44. The T cellular autoimmune response against immunodominant peptides of Dsg3 in patients with pemphigus vulgaris is monitored with highly specific HLA-DRbeta1*0402 tetramers. PMID: 17113829
45. These data indicate that Dsg3(dim) populations from primary human adult keratinocytes and long-term established keratinocyte lines possess certain stem/progenitor cell-like properties. PMID: 17255524
46. Data suggest that although the desmoglein (Dsg)3 depletion is not indicative for adhesive strength, it may indicate pathogenic changes within the cell and it plays a role in skin fragility or susceptibility to blister formation in pemphigus patients. PMID: 17428808
47. Reduction of Dsg3 might be relevant to blister formation in pemphigus vulgaris. PMID: 17431647
48. pemphigus vulgaris-IgG-dependent uPA activation is not related to anti-Dsg3 antibody activity PMID: 17532189
49. Autoantibody from pemphigus vulgaris sera react with non-conformational epitopes of desmoglein 3. PMID: 18095943
50. results indicate that the membrane proximal region in the IA region of Dsg3 is necessary for complex formation with P120-catenin and to maintain free Dsg3 at the cell surface before it is integrated into desmosomes PMID: 18343367
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亞細(xì)胞定位：

Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome.
組織特異性：

Epidermis, tongue, tonsil, esophagus and carcinomas.
數(shù)據(jù)庫鏈接：

HGNC: 3050

OMIM: 169615

KEGG: hsa:1830

STRING: 9606.ENSP00000257189

UniGene: Hs.1925

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