CD146 Recombinant Monoclonal Antibody

說明書

中文名稱：

CD146重組抗體
貨號：

CSB-RA013563A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in A375 whole cell lysate
All lanes CD146 antibody at 0.6μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72 KDa
Observed band size: 120 KDa

IHC image of CSB-RA013563A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of U251 cells with CSB-RA013563A0HU at 1:37,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Overlay histogram showing A375 cells stained with CSB-RA013563A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA013563A0HU CD146重組單克隆抗體是針對跨膜糖蛋白CD146（MCAM）研發的高特異性試劑，該靶點在細胞黏附、血管生成及免疫調節中發揮重要作用，尤其與腫瘤轉移和炎癥反應密切相關。本產品經嚴格驗證，適用于多種實驗場景：在蛋白質印跡（WB）中可清晰識別天然構象的CD146，推薦稀釋范圍為1:500至1:5000；免疫組化（IHC）實驗能有效定位組織樣本中的靶蛋白表達，建議使用1:50至1:500稀釋梯度；免疫熒光（IF）和流式細胞術（FC）分別可實現細胞水平的高分辨率定位及表面標志物定量分析，對應推薦稀釋比為1:30-1:200。其卓越的批間穩定性和低交叉反應特性，使其成為研究腫瘤微環境、血管生成機制及免疫細胞相互作用的高效工具，廣泛應用于細胞培養模型分析、冰凍/石蠟包埋組織切片染色以及原代細胞表面標記檢測等科研領域，為探索CD146在病理生理過程中的分子調控網絡提供可靠支持。
Uniprot No.：

P43121
基因名：

MCAM
別名：

A32 antigen antibody; CD 146 antibody; CD146 antibody; CD146 antigen antibody; Cell surface glycoprotein MUC18 antibody; Cell surface glycoprotein P1H12 antibody; Gicerin antibody; Mcam antibody; Melanoma adhesion molecule antibody; Melanoma associated antigen A32 antibody; Melanoma associated antigen MUC18 antibody; Melanoma associated glycoprotein MUC18 antibody; Melanoma cell adhesion molecule antibody; Melanoma-associated antigen A32 antibody; Melanoma-associated antigen MUC18 antibody; MelCAM antibody; MUC 18 antibody; MUC18 antibody; MUC18_HUMAN antibody; S endo 1 antibody; S endo 1 endothelial associated antigen antibody; S-endo 1 endothelial-associated antigen antibody
反應種屬：

Human
免疫原：

A synthesized peptide
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

8A10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500
IF	1:30-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
基因功能參考文獻：
1. MCAM coordination of apical-basal polarity and planar cell polarity provides insight into the general mechanisms of morphogenesis. PMID: 28589943
2. CD146 suppresses BC progression as a target of CD44-downstream signaling PMID: 29121955
3. Cultured early passage ASCs contained low levels of CD146 mRNA, which was expressed in two different splicing variants, at a relatively high amount of the CD146-long form and at a relatively low amount of the CD146-short form. ASCs contained low levels of CD146 protein, which consisted predominantly long form and a small amount of short form. PMID: 28549249
4. In summary, we have shown that MUC18/Muc18 may serve as a regulator of airway inflammation and mucus overproduction, two important features of type 2-high asthma. Our data suggest that MUC18/Muc18 or its downstream signaling mediators may be targets for potential therapeutic agents. PMID: 28451734
5. CD146 promoter polymorphisms were not associated with the risk of clear cell renal carcinoma in Chinese population. The rs3923594 was an independent predictor of recurrence in with localized ccRCC. PMID: 28626293
6. We demonstrate that ER(+) breast cancers contain two CAF subtypes defined by CD146 expression. CD146(neg) CAFs suppress ER expression in ER(+) breast cancer cells, decrease tumor cell sensitivity to estrogen, and increase tumor cell resistance to tamoxifen therapy PMID: 27702820
7. CD146 functions as a suppressor of tumorigenesis and cancer stemness in CRC through inactivating the canonical Wnt/beta-catenin cascade PMID: 27302922
8. data identify the anatomy and phenotype of a novel class of committed myogenic progenitor in human post-natal skeletal muscle of subendothelial cells associated with the abluminal surface of microvascular compartment distinct from satellite cells PMID: 29186180
9. These findings identify CD146 as a novel retention signal that traps macrophages within the artery wall, and a promising therapeutic target in atherosclerosis treatment. PMID: 28084332
10. CD146 was expressed in all cases of Ph-positive B- cell acute lymphoblastic leukemia and in the vast majority of T-cell acute lymphoblastic leukemia PMID: 26102234
11. Our results suggest that MCAM may serve as a novel therapeutic target to overcome chemoresistance in SCLC. PMID: 28646020
12. Authors show that KDM3A regulates MCAM expression both through a direct mechanism, involving modulation of H3K9 methylation at the MCAM promoter, and an indirect mechanism, via the Ets1 transcription factor. PMID: 28319067
13. increment of CD146 expression indicates gradual change of cultured annulus fibrosus cells to express a contractile phenotype and that transforming growth factor beta1 enhances this cellular commitment PMID: 27273299
14. CD146 positivity in immunohistological analysis of 11 MRT patient samples was associated with poor patient outcomes. These results suggest that CD146 defines a distinct sub-population in MRT with high tumorigenic capacity and that this marker represents a promising therapeutic target. PMID: 27041577
15. Promoter methylation of MCAM, ERalpha and ERbeta have a potential to be utilized as biomarker for the early detection of prostate cancer (PC) as their sensitivity and specificity seem to be better than serum PSA. PMID: 28147335
16. MCAM promotes tamoxifen resistance by transcriptionally suppressing ERalpha expression and activating the AKT pathway, followed by induction of epithelial-mesenchymal transition. PMID: 27838413
17. High MCAM expression is associated with lung metastasis in malignant melanoma. PMID: 27151304
18. Results provide evidence that MUC18 promotes viral infections both in vivo and in vitro. PMID: 27701461
19. soluble CD146 is released from the peripheral vasculature in response to venous stretch and may reflect systemic congestion in chronic heart failure patients PMID: 28062630
20. results indicate that CD146 can be targeted in vivo by the radiolabeled OI-3 antibodies PMID: 27776176
21. Findings suggest that decreased CD146 expression in cancer-associated fibroblasts promotes pancreatic cancer progression. PMID: 26373617
22. METCAM/MUC18 positively promotes tumorigenesis of human breast cancer SK-BR-3 cells via increasing the signal in survival and proliferation pathways. PMID: 27125403
23. sCD146 levels are elevated in patients with systemic sclerosis, but decreased sCD146 levels are observed in SSc patients with pulmonary arterial hypertension PMID: 27726047
24. Nestin and CD146 are expressed in breast cancer cells with highly aggressive potency. They might contribute to disease relapse in breast cancer by activating the epithelial-mesenchymal transition pathway and assist tumor neovascularization. PMID: 28347241
25. The results demonstrated isolation of specific scFv with frequency of 40 % which showed significant binding with the epitope in both ELISA and fluorescence-activated cell sorting (FACS) analyses. The antibody inhibited the migration (76 %) and invasion (67 %) of MUC18 positive cell line. The results suggest the specific anti-MUC18 scFv as an effective antibody for breast cancer immunotherapy PMID: 27565656
26. We provide the first report that pro-angiogenic genes PECAM1, PTGS1, FGD5, and MCAM may play a vital role in pathological dermal angiogenesis disorders of psoriasis. PMID: 26748901
27. Results showed that increased human METCAM/MUC18 expression in ovarian cancer SK-OV-3 cells suppressed tumorigenesis and ascites formation in nude mice, suggesting that human METCAM/MUC18 plays a suppressor role in the progression of ovarian cancer, perhaps by reducing proliferation and angiogenesis. PMID: 26906545
28. CD146 dexpression efines a subpopulation of human mesenchymal stem cells capable of bone formation and in vivo trans-endothelial migration. PMID: 26753846
29. Results showed that CD146 promoted metastasis of hepatocellular carcinoma (HCC) cells and predicted poor prognosis of HCC patients. CD146 induced epithelial mesenchymal transition through probably IL-8 up-regulation and STAT1 down-regulation. PMID: 26928402
30. Data indicate that CD146 antigen is an effective cell surface marker for enriching tumor-propagating cells (TPCs) in primary sarcomas. PMID: 26517673
31. MUC18 is an independent prognostic factor for clear cell renal cell carcinoma PMID: 26617818
32. CD146 is a novel and useful marker for predicting senescence in human umbilical cord blood-derived Mesenchymal stem cells (hUCB-MSCs), and CD146 can potentially be applied in quality-control assessments of hUCB-MSC-based therapy. PMID: 26941359
33. ZBTB7A directly binds to the promoter and transcriptionally represses the expression of MCAM, establishing ZBTB7A as a bona fide transcriptional repressor of MCAM PMID: 25995384
34. The expression of CD146 and HIF1a was positively correlated with EGFR and CD31, respectively in salivary gland adenoid cystic carcinoma. PMID: 25997612
35. a model that CD166 regulates MCAM through a signaling flow from activation of PI3K/AKT and c-Raf/MEK/ERK signaling to the inhibition of potential MCAM ubiquitin E3 ligases, betaTrCP and Smurf1 PMID: 26004137
36. Combined EpCAM/MCAM CellSearch enrichment thus increased the CTC detection rate. PMID: 25552367
37. Results identified MCAM as a novel YAP target in hepatocellular carcinoma (HCC) but not in breast and colon cancer cells. MCAM serum levels were specifically elevated in HCC suggesting it as a specific diagnostic tool for HCC. PMID: 25728681
38. CD146, P53, and Ki-67 are overexpressed in uterine sarcoma PMID: 26293576
39. These data suggest CDCP1 expression can be used to identify a subset of marrow fibroblasts functionally distinct from CD146+ fibroblasts. PMID: 25275584
40. Results show that that CD146 is expressed in 41% of gastric neoplasm cells and correlated positively with lymph node metastasis and epithelial-mesenchymal transition markers making it a good prognostic factor. PMID: 22754372
41. MCAM is a major GAL-1 ligand is largely dependent on melanoma malignancy. PMID: 25756799
42. MCAM is expressed by effector CD8+ T lymphocytes and it is strikingly upregulated during multiple sclerosis relapses. MCAM blockade restricts the transmigration of CD8(+) T lymphocytes across human blood-brain barrier endothelial cells. PMID: 25869475
43. HuMETCAM/MUC18 levels in ovarian carcinomas and metastatic lesions were significantly higher than in normal tissues and cystadenomas. PMID: 25510693
44. In peripheral stenotic arteriosclerotic disease the proangiogenic potency of MUC18 may play a role in angiogenesis of collaterals, whereas in dilatative aortic diseases the induction of collaterals is typically not evident. PMID: 25729916
45. Suggest endothelial CD146 as a target for specific drug delivery in hepatocellular carcinoma. PMID: 25238265
46. Data proposed a novel signaling mechanism by which Sema 3A regulates PTEN, FOXO 3a and MelCAM in a coordinated manner that leads to suppression of breast cancer growth and angiogenesis. PMID: 24727891
47. Authors conclude therefore that ETs upregulate MCAM in an Akt and ERK/MEK-dependent, but CREB-independent manner, providing an understanding for possible pharmacologic intervention in progressing melanoma. PMID: 24743054
48. expression behaves as a molecular warning of melanoma progression PMID: 24902661
49. With the ability of migration and survival in the advanced osteoarthritis cartilage, CD146+ chondroprogenitors might be "tissue-specific" for cartilage tissue regeneration. PMID: 25266708
50. functional characterization of N-acetylglucosaminyltransferases III and V in human melanoma cells PMID: 24726881
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亞細胞定位：

Membrane; Single-pass type I membrane protein.
組織特異性：

Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progress
數據庫鏈接：

HGNC: 6934

OMIM: 155735

KEGG: hsa:4162

STRING: 9606.ENSP00000264036

UniGene: Hs.599039

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