CD34 Recombinant Monoclonal Antibody

說明書

中文名稱：

CD34重組抗體
貨號：

CSB-RA004926A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA004926A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CD34重組單克隆抗體（CSB-RA004926A0HU）是針對細胞表面糖蛋白CD34研發的高特異性檢測工具。CD34作為重要的細胞標記物，廣泛表達于造血祖細胞、血管內皮細胞及間充質干細胞，參與細胞黏附和信號傳導調控，是造血系統發育、血管生成及組織再生研究的關鍵靶標。本抗體經嚴格驗證，在免疫組化（IHC）實驗中顯示優異性能，可在1:50-1:500稀釋范圍內實現靶蛋白的特異性定位染色，與CD34的跨膜表達特征高度吻合，適用于石蠟包埋組織等多種樣本類型；ELISA檢測體系驗證顯示其具備高親和力與低交叉反應性，滿足定量分析需求。該產品可廣泛應用于科研領域，包括造血干細胞鑒定、血管內皮細胞譜系追蹤、腫瘤微環境中血管新生的機制研究，以及再生醫學中干細胞標記與功能探索，為相關疾病的分子機制解析提供可靠工具。
Uniprot No.：

P28906
基因名：

CD34
別名：

CD34; Hematopoietic progenitor cell antigen CD34; CD antigen CD34
反應種屬：

Human
免疫原：

A synthesized peptide
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

1E7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:500

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
基因功能參考文獻：
1. This study aimed to confirm and analyze lymphovascular invasion (LVI) detected by hematoxylin and eosin (H and E) using IHC with CD34 and D2-40 and its correlation with other biologic and morphologic prognostic markers. D2-40 is a promising marker for lymphatic endothelium. LVI is a poor prognostic marker hence should be evaluated imperatively in all cases of carcinoma breast. PMID: 29567882
2. The role of CD34 in determining the premalignant nature of ral submucous fibrosis (OSF) could not be ascertained, since all endothelial cells were positive for CD34, whereas CD105 appeared to be more specific as it is associated with hypoxia-induced angiogenesis which is occurring in OSF due to hyalinization suggesting, CD105 to be more specific marker to determine neoangiogenesis in OSF PMID: 30197335
3. EZH2, ET-1, and CD34 may act as biomarkers of aggressive cervical squamous cell carcinoma PMID: 29630085
4. The effects of Platelet-rich plasma on vascular endothelial growth factor receptor-2 (VEGFR2) and CD34 expression were evaluated using real-time PCR, flow cytometry, western blot, immunocytochemistry and pathological study, as were carried out in both human umbilical endothelial cell culture and rat skin PMID: 28948378
5. Disruption of GFI1B non-DNA-binding zinc-finger 1 is associated with mild to moderate thrombocytopenia without alpha-granule deficiency or bleeding symptomatology, indicating that the site of GFI1B mutation has important phenotypic implications. Platelet CD34 expression appears to be a common feature of perturbed GFI1B function. PMID: 28880435
6. The endometrial expression of LIF and CD34 in the pathogenesis of non-developing pregnancy can be used for evaluating the pregnancy prognosis in women of young and old reproductive age. PMID: 29063331
7. In summary, this study shows that CD34 plays a role during cell cycle progression of head and neck squamous cell carcinoma and additionally is involved in irradiation resistance and differentiation of malignant oral keratinocytes. PMID: 27837456
8. Report CD34 expression in dermatofibromas and dermatofibrosarcoma protuberans. PMID: 28832078
9. CD34+ clusters showed more important prognostic impact on survival and progression to leukaemia. PMID: 28669578
10. Our findings showed that increased expression of CD34 and CD117 markers confer tumor progression and aggressiveness on prostate cancer. PMID: 28552539
11. CD34(+) and CD34(-) cells are different phenotypes of similar cells, and CD34 (1) can be regulated in endothelial colony-forming cells ECFC; (2) is positively involved in capillary-like sprout formation; (3) is associated but not causally related to tip-cell gene expression; and (4) can affect endothelial barrier function. PMID: 27043316
12. Studied levels of peripheral blood CD34(+ )cells in women who subsequently had a spontaneous miscarriage (SM). Found CD34 positive B cells were significantly lower in SM group compared to control group. PMID: 28110587
13. The number of platelets and circulating CD34-positive cells acts as an indicator of the activity of the vicious cycle that exists between hypertension and endothelial dysfunction in elderly Japanese men. PMID: 28282559
14. ESAT-6, an essential virulence factor of Mycobacterium tuberculosis, may contribute to the abnormal hematopoiesis of tuberculosis patients by inhibiting the proliferation and differentiation of CD34-positive hematopoietic cells via apoptosis. PMID: 27745787
15. Intramyocardial autologous CD34(+) cell administration improved exercise tolerance in patients with refractory angina. PMID: 27491607
16. Increased gene expression linked to the proinflammatory TGFbeta and MAPK signaling pathways were detected in CD34(+) cells of myeloproliferative neoplasm patients. PMID: 27341002
17. CD34 stem cell exosomes mediate ischemic tissue repair via beneficial angiogenesis. Exosome-shuttled proangiogenic miRNAs may amplify stem cell function. PMID: 28298297
18. miR-378 regulates the angiogenic capacity of CD34(+) progenitor cells in STEMI patients. PMID: 28062497
19. CD34 and IL-8 are associated with poor prognosis of melanoma, metastasis, and neoangiogenesis PMID: 27175552
20. The results showed that transfection of CD34(+) cells with SiRNA targeting TGF-bRII and their co-culture with human bone marrow mesenchymal stromal cells (MSCs) could considerably increase the number of progenitors PMID: 27344285
21. Expression of CD34 and MMP-9 accurately predicts clinical behavior detection and prognosis of GCTBs. PMID: 27384731
22. Expression of VEGF and CD34 was critically correlated with perioperative hemorrhage in gastric cancer patients. PMID: 27503021
23. There was significant correlation between age and tumor size with CD34 expression in oral SCC samples and no significant correlation between sex and tumor differentiation level. Also, there was no significant correlation between age, sex, tumor size and tumor differentiation level (grading) with CD34 expression in esophagus SCC samples. PMID: 26323448
24. Normal FLT3 and negative expression of CD34 and cMPL may predict a longer overall survival in aute myeloid leukemia. PMID: 27993871
25. High VEGF expression was subsequently correlated with a short overall survival rate for patients exhibiting lymph node metastasis (P=0.0128); however, there was no significant difference in overall survival rate regarding the expression levels of TP and CD34 PMID: 26676225
26. Metformin simultaneously increases VEGFA and reduces CXCL10 and TIMP1 in CD34(+) cells in a model of the diabetic state combined with hypoxia to stimulate angiogenesis. PMID: 26861446
27. Immunohistochemistry results indicate that combination with ALDH1 and STAT6 can improve the diagnostic value of CD34 for solitary fibrous tumors. PMID: 26617768
28. CD34(+) cells lowering expression of TET2 may play an oncogenic role on myeloid tumor and CD3(+) T cells of myelodysplastic syndrome patients may be derived from the malignant clone. PMID: 26617797
29. ICAM-1, VCAM-1 and CD34 are useful biomarkers in evaluation of vascular and inflammatory lesions such as gingival pyogenic granuloma and the results indicate the role of these biomarkers in pathogenesis of oral pyogenic granuloma. PMID: 26625754
30. Studied the relationship between serum alkaline phosphatase, circulating CD34-positive cells and body mass index. PMID: 26769093
31. Autophagy in CD34+ cells from aplastic anemia patients is impaired; autophagy is required for the survival of CD34+ cells; impaired autophagy in CD34+ hematopoietic progenitor cells may play an important role in the pathogenesis of aplastic anemia. PMID: 26930650
32. overexpression in glioma tissues associated closely with higher WHO grade (III + IV) [review] PMID: 26886640
33. Asthmatics with reduced lung function had a higher frequency of Lin(-) CD34(hi) CD117(int/hi) Fc epsilon RI(+) blood mast cell progenitors than asthmatics with normal lung function. PMID: 26626992
34. Our results suggest that the CD34/CD25/CD123/CD99(+) LAIP is strictly associated with FLT3-ITD-positive cells. PMID: 25957287
35. Data indicate only the glycoproteins CD34+/CD71low population showed statistically significant differences between Diamond-Blackfan Anaemia (DBA) patients and controls. PMID: 26394034
36. We here identify CD49f and CD34 as markers to permit selection of the stem/progenitor cell-like population from human myometrium PMID: 26108791
37. CD34 expression in the placental endothelial cells was stable throughout pregnancy. PMID: 25043745
38. Autologous CD 34(+) cell infusion is safe and effectively improves liver function in the short term and may serve as a bridge to liver transplantation. PMID: 26109814
39. Higher percentages of CD34 + CD38- cells in apheresis products reflect inadequate in vivo purging and reliably distinguish samples enriched in LSCs from those involving mainly normal cells. PMID: 24884314
40. The number of CD34/144 positive circulating endothelial cells was high in patients with large artery atherosclerosis who experienced chronic ischemic stroke. And this number may reflect severity of carotid atherosclerosis. PMID: 25542764
41. The evaluation of both CD34-CD105 showed the role of angiogenesis in the cancer proliferation and local spread, the angiogenesis level being maintained high even in the advanced stages of the disease. PMID: 26193208
42. There were significant differences in CD34 expression between papillary thyroid hyperplasia, papillary thyroid carcinoma, and papillary thyroid lesions of uncertain malignant potential. PMID: 25921136
43. lack of predictive factors for CD34(+) cells and total nucleated cell concentrations in umbilical cord blood samples before processing PMID: 25446160
44. The number of CD34(+) cells in bone marrow was significantly higher compared to the number of CD34(+) cells in peripheral blood cells and increased with the acquisition of leukemic cells the resistance to imatinib. PMID: 25804236
45. CD34 and CD49b are cell surface markers that can be used to enrich a subpopulation of leiomyoma cells possessing stem/progenitor cell properties. PMID: 25658015
46. no difference in expression between normal and pathologically immature placentas PMID: 25149387
47. Report CD34/MMP7 expression and invasiveness in early colorectal neoplasms using narrow band imaging and immunohistochemistry. PMID: 25357219
48. Angiogenic factors VEGF, HIF-1alpha and CD34 are important in breast cancer progression and their abundance in breast tumors has prognostic and predictive value. PMID: 23899963
49. Combinatorial expression of NKX2-5, SIRPA, VCAM1 and CD34 can be used to define discrete stages of cardiovascular cell lineage differentiation. PMID: 24968096
50. In MLL-AF4 patients, CD34(+)CD38(+)CD19(+) and CD34(-)CD19(+) cells initiated leukemia. PMID: 25538041
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亞細胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

CD34 family
組織特異性：

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
數據庫鏈接：

HGNC: 1662

OMIM: 142230

KEGG: hsa:947

STRING: 9606.ENSP00000310036

UniGene: Hs.374990

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