CD40

CD40，即腫瘤壞死因子受體超家族成員5（TNFRSF5），別名Bp50、CD40L等。CD40由人類CD40基因編碼，廣泛表達(dá)于免疫細(xì)胞，特別是B細(xì)胞、樹突狀細(xì)胞（DC）和單核細(xì)胞。CD40是一種單鏈型跨膜蛋白，包括一個(gè)胞外區(qū)域、一個(gè)跨膜區(qū)域和一個(gè)胞漿區(qū)域，其胞外區(qū)域可以與其配體CD40L（CD40配體）結(jié)合，觸發(fā)信號(hào)傳導(dǎo)。CD40在連接先天免疫和適應(yīng)性免疫方面發(fā)揮著重要作用，其功能擴(kuò)展到B細(xì)胞激活、抗原呈遞和炎癥調(diào)節(jié)等多個(gè)層面。CD40對(duì)于體液免疫應(yīng)答、免疫記憶的形成以及免疫調(diào)節(jié)都具有重要意義。CD40信號(hào)傳導(dǎo)也在免疫治療研究中被廣泛研究，以開發(fā)新的治療方法來增強(qiáng)或抑制免疫系統(tǒng)的特定響應(yīng)。

熱銷產(chǎn)品

Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active) (CSB-MP004936HU1)

驗(yàn)證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized CD40 at 2 μg/ml can bind CD40L (CSB-MP004937HU3), the EC₅₀ is 3.112-3.858 ng/ml.

Activity
Human CD40 protein hFc tag (CSB-MP004936HU1) captured on COOH chip can bind Human CD40L protein hFc and Flag tag (CSB-MP004937HU3) with an affinity constant of 2.06 nM as detected by LSPR Assay.

The purity of CD40 was greater than 95% as determined by SEC-HPLC

CD40 Recombinant Monoclonal Antibody (CSB-RA004936MA1HU)

驗(yàn)證數(shù)據(jù)

Immunofluorescence staining of A549 cell with CSB-RA004936MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-mouse IgG(H+L).

Immunofluorescence staining of PC-3 cell with CSB-RA004936MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-mouse IgG(H+L).

Overlay Peak curve showing THP-1 cells surface stained with CSB-RA004936MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,012 events was performed.

CD40 Antibody

CD40 Protein

CD40 ELISA Kit

CD40 Antibodies

CD40 for Homo sapiens (Human)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA004936GA01HU	CD40 Antibody	Human	ELISA,WB
CSB-PA176134	Phospho-CD40 (Thr254) Antibody	Human,Mouse	ELISA,WB
CSB-PA001455	CD40 Antibody	Human,Mouse,Monkey	WB, IHC, ELISA
CSB-PA080031	Phospho-CD40 (T254) Antibody	Human,Mouse	WB, ELISA
CSB-PA215588	CD40 Antibody	Human,Mouse	ELISA,IHC
CSB-PA004936LA01HU	CD40 Antibody	Human, Mouse	ELISA, WB, IF
CSB-PA004936LB01HU	CD40 Antibody, HRP conjugated	Human	ELISA
CSB-PA004936LC01HU	CD40 Antibody, FITC conjugated	Human
CSB-PA004936LD01HU	CD40 Antibody, Biotin conjugated	Human	ELISA
CSB-RA004936MA1HU	CD40 Recombinant Monoclonal Antibody	Human	ELISA, IF, FC

CD40 Proteins

CD40 Proteins for Mus musculus (Mouse)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP004936MO1 CSB-EP004936MO1 CSB-BP004936MO1 CSB-EP004936MO1-B	Recombinant Mouse Tumor necrosis factor receptor superfamily member 5 (Cd40), partial	Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli
CSB-CF004936MO	Recombinant Mouse Tumor necrosis factor receptor superfamily member 5 (Cd40)	in vitro E.coli expression system

CD40 Proteins for Homo sapiens (Human)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP004936HU1 CSB-EP004936HU1 CSB-EP004936HU1-B	Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial	Yeast E.coli In Vivo Biotinylation in E.coli
CSB-CF004936HU	Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40)	in vitro E.coli expression system
CSB-AP005171HU	Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active)	Mammalian cell
CSB-AP005181HU	Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active)	Mammalian cell
CSB-MP004936HU1	Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active)	Mammalian cell
CSB-BP004936HU1	Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial	Baculovirus

CD40 Proteins for Bos taurus (Bovine)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP636713BO CSB-EP636713BO CSB-BP636713BO CSB-MP636713BO CSB-EP636713BO-B	Recombinant Bovine Tumor necrosis factor receptor superfamily member 5 (CD40), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40 Proteins for Callithrix jacchus (White-tufted-ear marmoset)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP659466CYL CSB-EP659466CYL CSB-BP659466CYL CSB-MP659466CYL CSB-EP659466CYL-B	Recombinant Callithrix jacchus Tumor necrosis factor receptor superfamily member 5 (CD40), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40 Proteins for Equus caballus (Horse)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP675871HO CSB-EP675871HO CSB-BP675871HO CSB-MP675871HO CSB-EP675871HO-B	Recombinant Horse Tumor necrosis factor receptor superfamily member 5 (CD40), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40 Proteins for Canis familiaris (Dog) (Canis lupus familiaris)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP767175DO CSB-EP767175DO CSB-BP767175DO CSB-MP767175DO CSB-EP767175DO-B	Recombinant Dog Tumor necrosis factor receptor superfamily member 5 (CD40), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40 Proteins for Sus scrofa (Pig)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-BP851448PI CSB-MP851448PI CSB-EP851448PI-B	Recombinant Pig Tumor necrosis factor receptor superfamily member 5 (CD40), partial	Baculovirus Mammalian cell In Vivo Biotinylation in E.coli