CD163 Recombinant Monoclonal Antibody

說明書

中文名稱：

CD163 Recombinant Monoclonal Antibody
貨號：

CSB-RA801238MA1HU
規格：

￥1320
圖片：
IHC image of CSB-RA801238MA1HU diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CD163重組單克隆抗體（CSB-RA801238MA1HU）是針對巨噬細胞特異性表面標志物CD163開發的高特異性科研試劑。CD163作為血紅蛋白-觸珠蛋白復合體的清道夫受體，主要在抗炎型巨噬細胞中高表達，其表達水平與多種炎癥性疾病及腫瘤微環境調控密切相關。本產品經ELISA和免疫組化（IHC）雙重驗證，在IHC應用中推薦使用1:50-1:200稀釋度，可清晰顯示組織樣本中巨噬細胞的膜定位信號，背景干擾低且批次穩定性優異。適用于石蠟包埋組織切片的免疫標記，能夠有效區分M2型巨噬細胞亞群，為研究腫瘤相關巨噬細胞浸潤、慢性炎癥微環境調控及巨噬細胞功能異質性提供可靠工具。在腫瘤免疫學研究中，可用于分析腫瘤組織內免疫細胞組成；在神經退行性疾病模型中，可輔助探究小膠質細胞活化狀態；在代謝性疾病領域，有助于評估脂肪組織巨噬細胞的極化特征。該抗體為探索巨噬細胞在病理生理過程中的作用機制及潛在治療靶點研究提供了優質試劑選擇。
Uniprot No.：

Q86VB7
基因名：

CD163
別名：

C163A_HUMAN antibody; CD 163 antibody; CD163 antibody; CD163 antigen antibody; CD163 molecule antibody; Hemoglobin scavenger receptor antibody; M130 antibody; M130 antigen precursor antibody; Macrophage associated antigen antibody; MM130 antibody; OTTHUMP00000238617 antibody; OTTHUMP00000238618 antibody; OTTHUMP00000238619 antibody; OTTHUMP00000238620 antibody; SCARI1 antibody; Scavenger receptor cysteine rich type 1 protein M130 antibody; sCD163 antibody; Soluble CD163 antibody
反應種屬：

Human
免疫原：

Recombinant Human CD163 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

hIgG1
純化方式：

Affinity-chromatography
克隆號：

26F9
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.; After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
基因功能參考文獻：
1. high plasma levels of sCD163 is associated with measles virus infection. PMID: 29795672
2. Increased sCD163 levels in sputum are associated with the impairment of lung function in asthma patients PMID: 30246699
3. Expression of CD163 in urinary bladder cancer cells is significantly correlated with macrophage infiltration and might have a tumor promoting impact. PMID: 29288002
4. A separate measurement of the two forms of CD163 constituting 'soluble CD163' in plasma may therefore add to the diagnostic and prognostic value. PMID: 28084321
5. our results suggest that MR and CD163 may be novel biomarkers for CRC detection and survival evaluation. PMID: 29226859
6. Data suggest a novel pathophysiological role of CD163 in type 2 diabetes; monocyte surface CD163 levels are significantly associated with insulin resistance in patients with type 2 diabetes; the association of insulin resistance with soluble CD163 levels is less significant. This study was conducted in Japan. PMID: 29445750
7. Detection of the CD163 antigen on cancer cells might be a useful marker for evaluating the clinical course of patients with clear cell renal cell carcinoma PMID: 28687956
8. CD163-expression by cancer cells was significantly associated with MI and clinicopathological data. Patients with CD163-positive tumors had significantly shorter disease-free survival (DFS) after RT. PMID: 29725763
9. Plasma CD163 levels were increased in obese and type 2 diabetes mellitus patients compared to controls. PMID: 28406702
10. soluble CD163 is associated with markers of liver necro-inflammation and glucose homoeostasis in non-alcoholic fatty liver disease. PMID: 28715286
11. The effect of LPS in the expression of CD163 and CD206 on monocytes is not reverted in LPS tolerant cells, and the inhibition of inflammatory cytokines in tolerant cells is not related with modulation of these receptors PMID: 26352275
12. The cumulative incidence of de novo-onset cGVHD was higher in patients with higher plasma soluble CD163 concentrations at day 80 than those with lower concentrations (75% versus 40%, P = .018). The cumulative incidence of de novo- or quiescent-onset cGVHD did not differ statistically according to concentrations of the 3 other proteins at day 80. PMID: 28455006
13. Although sCD163 level cannot predict the development of varices, it serves as a good predictor for the detection of size of varices (large varices), the need of prophylactic interventions, and risk of variceal bleeding. sCD163 level is a helpful indicator with the progression of cirrhosis and portal hypertension. PMID: 28282272
14. CD163 and ferritin are biomarkers of functional outcomes in intracerebral hemorrhage. PMID: 28392117
15. the simultaneous detection of both plasma sCD14 and IL-6 is a promising diagnostic approach to identify active pulmonary tuberculosis, and further, measurement of TNF-alpha and sCD163 can identify the most severe cases of tuberculosis. PMID: 27591510
16. The macrophage activation marker CD163 was increased in patients with active pulmonary TB compared with age- and sex-matched control subjects. PMID: 27684274
17. alveolar macrophages from asthmatic subjects have reduced cell-surface expression of CD163, which suggests that CD163 might modulate the pathogenesis of asthma PMID: 26376364
18. Serum sCD163 might be a potential biomarker for predicting the severity and prognosis of PM/DM-related interstitial lung disease PMID: 28103926
19. Soluble CD163 reflecting macrophage activation is associated with morphological features of non-alcoholic fatty liver disease. An independent association between sCD163 and cytokeratin-18 suggests that apoptosis may contribute to macrophage activation in NAFLD/NASH. PMID: 27102725
20. Most cases of histiocytic sarcoma expressed histiocytic markers CD68 (6 of 7 cases), CD163 (5 of 5 cases), and PU.1 (3 of 4 cases). PMID: 28805986
21. Within a diabetic cohort, the percentage of monocytes expressing CD163 was decreased in those subjects with complications, compared to those without complications. These changes were not accompanied by a change in gene expression. Glucocorticoids increased CD163 expression in cultured peripheral blood monocytes from non-diabetic persons. PMID: 27354410
22. sCD163 was markedly elevated in autoimmune hepatitis (AIH) in the acute phase, normalised by successful treatment in complete responders, but remained higher in the incompletely responding cases. Our results demonstrate macrophage activation in AIH paralleling disease activity, severity and treatment response, suggesting a role for macrophage activation in AIH. PMID: 27679428
23. High CD163 expression is associated with glomerular inflammation in lupus nephritis. PMID: 27242373
24. analysis of plasma CXCL10, sCD163 and sCD14 in virological suppression and risk of cardiovascular disease PMID: 27355513
25. we found that higher percentages of circulating CD14+CD204+, CD14+CD163+CD204+ M2-like monocytes were significantly associated with TNM stage, lymph node metastasis, and histological differentiation. PMID: 28639912
26. CD163 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
27. Soluble CD163, which is identified as a marker of inflammation and type II diabetes, is elevated in polycystic ovary syndrome. PMID: 26985764
28. soluble CD163 modulates the immune response to Dermatophagoides pteronyssinus allergens potentiating anti-inflammatory, homeostatic mechanisms. PMID: 27100336
29. The mean serum-soluble CD163 level was higher in the ANCA-associated renal vasculitis patients with infectious complications than in the active-vasculitis patients, inactive-vasculitis patients, and normal controls. PMID: 27094919
30. we have developed a highly sensitive targeted probe capable of detecting CD163-expressing macrophages that could provide useful information about the state of the atheromatous lesions. PMID: 26616677
31. A subset of CD163+ macrophages displays mixed polarizations in discoid lupus skin. PMID: 26568320
32. Increased soluble CD163 blood levels in hepatocellular carcinoma patients correlates with rapid hepatocellular carcinoma progression. PMID: 26549495
33. these data indicate that sCD163 is a prognostic marker in B-CLL. PMID: 25747973
34. sCD163 is a sensitive marker protein for liver failure. PMID: 26339412
35. CD163-positive macrophages predict outcome in follicular lymphoma, but their prognostic impact is highly dependent on treatment received. PMID: 25869385
36. therapy with rituximab, cyclophosphamide and fludarabine resulted in a significant reduction in the number of non-classical CD14+CD16++ monocytes and soluble form of CD163 but upregulation of membrane-associated monocyte CD163 PMID: 26135617
37. Renal graft CD163+ infiltrates correlated strongly with interstitial inflammation, tubulitis, and peritubular capillaritis. PMID: 26087825
38. Higher levels of circulating monocyte-platelet aggregates are correlated with viremia and increased sCD163 levels in HIV-1 infection. PMID: 25109683
39. CD-163, related to immune-system (macrophage), correlated with symptoms (pain or discomfort) of prostatic inflammation. PMID: 26045748
40. The correlation of CD antigens with cytomegalovirus (CMV) antibodies in HIV positive people showed that increased CD163 but not CD14 is a marker of CMV infection. PMID: 26569178
41. Soluble CD163 is increased in patients with acute pancreatitis. PMID: 26209500
42. CD163 may be correlated with disease severity and the disease progression in patients with hemorrhagic fever with renal syndrome; however, the underlying mechanisms should be explored further. PMID: 25392926
43. Suggest MelanA-negative spindle-cell associated melanoma constitutes a distinct inflammatory phenotype correlated with dense infiltration of CD163 macrophages and loss of E-cadherin. PMID: 25602697
44. The scavenger receptor CD163 has been found to be over-expressed in Head and neck squamous cell carcinoma. PMID: 24858806
45. Show a high percentage of CD163+ tumor-associated macrophages and a low percentage of S100+ dendritic cells in thymic carcinoma samples. PMID: 25499804
46. CD163 is a biomarker for the clinical assessment of macrophage proliferation and activity that would help in risk prediction of bone and lung involvement and monitoring treatment response PMID: 25587690
47. This study describe elevated soluble CD163 (sCD163)levels in gestational diabetes mellitus (GDM) and identify human placenta as a novel source of sCD163 suggesting that placental tissues might contribute to the increased levels of circulating sCD163 in GDM pregnancies. PMID: 24983948
48. could be potentially used as an immune diagnostic marker for malignant pleural effusion PMID: 25944005
49. Soluble CD163 may be a marker of "unhealthy" fat distribution in obese subjects. PMID: 25376508
50. IL-8 might be involved in poor clinical outcomes via generation of CD163-positive M2 macrophages, and these factors in addition to N status might have prognostic value in patients with resectable oral squamous cell carcinoma PMID: 25461761
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亞細胞定位：

[Soluble CD163]: Secreted.; Cell membrane; Single-pass type I membrane protein. Note=Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
組織特異性：

Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed a
數據庫鏈接：

HGNC: 1631

OMIM: 605545

KEGG: hsa:9332

STRING: 9606.ENSP00000352071

UniGene: Hs.504641

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